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The Proteomics Protocols Handbook

  • Book
  • © 2005

Overview

Editors:
  1. John M. Walker

    1. University of Hertfordshire, Hatfield, UK

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Part of the book series: Springer Protocols Handbooks (SPH)

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About this book

Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein. The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. All laboratory-based protocols follow the successful Methods in Molecular Biologyâ„¢ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.



   

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Table of contents (73 protocols)

  1. Front Matter

    Pages i-xviii
    Download chapter PDF

  2. Extraction and Solubilization of Proteins for Proteomic Studies

    • Richard M. Leimgruber
    Pages 1-18

  3. Preparation of Bacterial Samples for 2-D PAGE

    • Brian Berg Vandahl, Gunna Christiansen, Svend Birkelund
    Pages 19-26

  4. Preparation of Yeast Samples for 2-D PAGE

    • Joakim Norbeck
    Pages 27-30

  5. Preparation of Mammalian Tissue Samples for Two-Dimensional Electrophoresis

    • Frank A. Witzmann
    Pages 31-35

  6. Differential Detergent Fractionation of Eukaryotic Cells

    • Melinda L. Ramsby, Gregory S. Makowski
    Pages 37-48

  7. Serum or Plasma Sample Preparation for Two-Dimensional Gel Electrophoresis

    • Anthony G. Sullivan, Stephen Russell, Henry Brzeski, Richard I. Somiari, Craig D. Shriver
    Pages 49-54

  8. Preparation of Plant Protein Samples for 2-D PAGE

    • David W. M. Leung
    Pages 55-57

  9. Laser-Assisted Microdissection in Proteomic Analyses

    • Darrell L. Ellsworth, Stephen Russell, Brenda Deyarmin, Anthony G. Sullivan, Henry Brzeski, Richard I. Somiari et al.
    Pages 59-66

  10. Purification of Cellular and Organelle Populations by Fluorescence-Activated Cell Sorting for Proteome Analysis

    • William L. Godfrey, Colette J. Rudd, Sujata Iyer, Diether Recktenwald
    Pages 67-78

  11. Purification of Nucleoli From Lymphoma Cells and Solubilization of Nucleolar Proteins for 2-DE Separation

    • Régis Dieckmann, Yohann Couté, Denis Hochstrasser, Jean-Jacques Diaz, Jean-Charles Sanchez
    Pages 79-85

  12. Prefractionation of Complex Protein Mixture for 2-D PAGE Using Reversed-Phase Liquid Chromatography

    • Volker Badock, Albrecht Otto
    Pages 87-95

  13. Fractionation of Complex Proteomes by Microscale Solution Isoelectrofocusing Using ZOOM− IEF Fractionators to Improve Protein Profiling

    • Xun Zuo, Ki-Boom Lee, David W. Speicher
    Pages 97-117

  14. Large-Format 2-D Polyacrylamide Gel Electrophoresis

    • Henry Brzeski, Stephen Russell, Anthony G. Sullivan, Richard I. Somiari, Craig D. Shriver
    Pages 119-131

  15. Analysis of Membrane Proteins by Two-Dimensional Gels

    • Michael Fountoulakis
    Pages 133-144

  16. 2-D PAGE of High-Molecular-Mass Proteins

    • Masamichi Oh-Ishi, Tadakazu Maeda
    Pages 145-149

  17. Using Ultra-Zoom Gels for High-Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis

    • Sjouke Hoving, Hans Voshol, Jan van Oostrum
    Pages 151-165

  18. NEpHGE and pI Strip Proteomic 2-D Gel Electrophoretic Mapping of Lipid-Rich Membranes

    • Steven E. Pfeiffer, Yoshihide Yamaguchi, Cecilia B. Marta, Rashmi Bansal, Christopher M. Taylor
    Pages 167-175

  19. Silver Staining of 2-D Gels

    • Julia Poland, Thierry Rabilloud, Pranav Sinha
    Pages 177-184

  20. Zn2+Reverse Staining Technique

    • Carlos Fernandez-Patron
    Pages 185-192
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Editors and Affiliations

  • University of Hertfordshire, Hatfield, UK

    John M. Walker

Bibliographic Information

  • Book Title: The Proteomics Protocols Handbook

  • Editors: John M. Walker

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1385/1592598900

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2005

  • Hardcover ISBN: 978-1-58829-343-5Published: 09 March 2005

  • Softcover ISBN: 978-1-58829-593-4Published: 09 March 2005

  • eBook ISBN: 978-1-59259-890-8Published: 09 October 2007

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: XVIII, 988

  • Topics: Proteomics

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