Serum IgE Induced Airway Smooth Muscle Cell Remodeling Is Independent of Allergens and Is Prevented by Omalizumab
Fig 1
Dose and disease specific effects of serum and IgE on ASMC.
(A) Concentration-dependent proliferative effect of human versus fetal calf serum (FCS) on primary ASMC isolated from asthmatic (n = 5) and non-asthmatic (n = 5) patients. (B) Disease specific ASMC proliferation by 2% fetal calf serum or control human serum. (C) Disease and origin specific proliferative effect of four different serum groups (control, atopic-non asthma, asthma, atopic asthma) on asthmatic and non-asthmatic ASMC. Ten sera of each donor group were tested on 5 ASMC of asthma patients and 5 ASMC of non-asthma patients. (D) Inhibitory effect of Omalizumab on serum-induced proliferation on asthmatic ASMC (n = 5). (E) The role of Erk1/2 MAPK and Igε-RI on serum-induced proliferation of asthmatic ASMC. * indicates statistic significant reduction of asthmatic ASMC proliferation compared to serum stimulation (first black bar in each group) with p < 0.05. All presented values present mean±S.E.M.