Differentiated Type II Pneumocytes Can Be Reprogrammed by Ectopic Sox2 Expression
Figure 7
Primary alveolar type II cells can be reprogrammed in vitro.
Representative immunofluorescence images showing expression of iSox2 (Red), Cc10 (Green), Sca1(Green) and Trp63 (Green) after culturing the primary type II cells with doxycycline for 3 days (A), 6 days (B) and 9 days (C). AVTII cells cultured for 9 days without doxycycline served as negative controls (D). The results demonstrate that in vitro cultures of AVTII cells have comparable phenotypic plasticity as AVTII cells in vivo. Scale bar 50 µm (A–C), 100 µm (D).