Comparative Characteristics of Porous Bioceramics for an Osteogenic Response In Vitro and In Vivo
Figure 6
Results of radiopacity for the newly formed tissues after bioceramic implantation. Representative radiographs (A) and histological analysis (B) of a femur with bioceramics implantation for 12 weeks.
(A) Density of the bone was analyzed by X-ray, and radiopacity levels were measured at the same area (red rectangle) from the bioceramics implanted region to the opposite side. The graph shows the relative opacity of X-ray films, which indicates the kind of implanted bioceramics. The stronger the radiopaque image in the red dashed rectangle, the higher the bone density. It is clear that the CMP sample exhibits higher radiopacity than the others. Vetbond, HA, and HA-col groups were at the same relative opacity, which was higher than that in the control. *p<0.01 versus the control. (B) The cortical defect on the femur of all groups showed newly formed eosinophilic connective tissues. At higher magnification, the composition and density of the newly formed tissues of bioceramics were different from the control and Vetbond samples. Bioceramic-implanted groups revealed marked compact structures when compared to the others, which corresponds with the immunoreactivity of ALP and type I collagen. Representative H&E stained sections of bioceramic implants from 12 weeks after surgery. H&E, Magnification, ×100 and ×200. Immunohistochemistry for ALP and type I collagen, Mayer's hematoxylin counter staining, Magnification, ×200.