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Gene Transfection in High Serum Levels: Case Studies with New Cholesterol Based Cationic Gemini Lipids

Figure 6

Gel electrophoresis to find out DNA binding and release efficiency.

Electrophoretic gel patterns for the lipoplex-associated pEGFP-C3 plasmid DNA. (A) DNA binding efficiency of different gemini lipid based lipoplexes. The N/P ratios are indicated at the top of each lane. (B) SDS mediated DNA release from representative lipid based lipoplexes. The SDS/lipid ratios are indicated below each lane. Both experiments were performed using 0.2 µg of DNA per well.

Figure 6

doi: https://doi.org/10.1371/journal.pone.0068305.g006