Succinate Dehydrogenase Is a Direct Target of Sirtuin 3 Deacetylase Activity
Figure 3
Complex II (A) and acetylated proteins (B) were immunoprecipitated from liver mitochondria isolated from SIRT3 WT and KO mice. IPs were immunoblotted with antibodies against acetyl-lysine (AcK), SDHA, SDHB and GDH. (C) Complex II immunoprecipitated from mouse liver mitochondria was incubated with recombinant SIRT3 or catalytically inactive SIRT3 (SIRT3 H248Y) in the presence or absence of NAD and NAM, a sirtuin inhibitor. After deacetylation, IPs were immunoblotted using antibodies against acetylated proteins, SDHA and SIRT3. In all panels, antibodies against GFP were used as negative controls.