A Novel Immune Evasion Strategy of Candida albicans: Proteolytic Cleavage of a Salivary Antimicrobial Peptide
Figure 3
Degradation of Hst-5 under various conditions and C. albicans strains.
(A) Complete degradation of Hst-5 by proteinase K (PK) used as positive control and inhibition of degradation by PMSF but not by pepstatin A (P) (B) Conversely, inhibition of C. albicans degradation by pepstatin A but not by PMSF (C) Minimal degradation observed when Hst-5 was incubated with C. albicans supernatant (CA-sup) D) Significant degradation of Hst-5 by 0.0625 µg Sap9 and Sap10 purified proteases and inhibition of degradation by pepstatin A.