Kombucha Tea-associated microbes remodel host metabolic pathways to suppress lipid accumulation
Fig 2
KTMs restrict lipid accumulation in the host.
(A) Representative images (scale bar, 500 μm) and (B) quantification (mean ± SD, ****, P<0.0001, one-way ANOVA) of day 1 adults stained with Oil Red O. (C) Representative fluorescence images (scale bar, 500 μm) and (D) quantification (mean ± SD, ****, P<0.0001, one-way ANOVA) of day 1 adults stained with Nile Red. (E) Biochemical quantification of the triglycerides (TAGs per animal) in animals consuming each food source (mean ± SEM, ***, P<0.001, *, P<0.05, ns, not significant, one-way ANOVA). (F) Representative fluorescence images of DHS-3::GFP (dhs, dehydrogenase, short chain) at intestinal lipid droplets in animals consuming the indicated microbial diets (scale bar, 5 μm). (G) Lipid droplet size measurements with each datapoint representing the average intestinal lipid droplet diameter for a single animal (mean ± SD, ****, P<0.0001, one-way ANOVA). (H) Lipid droplet density measurements with each datapoint representing the number of lipid droplets per μm2 for a single animal (mean ± SD, ****, P<0.0001, *, P<0.05, ns, not significant, one-way ANOVA). Raw data underlying panels B, D, E, G, and H can be found in S2 Data.