Glial immune-related pathways mediate effects of closed head traumatic brain injury on behavior and lethality in Drosophila
Fig 6
Post-TBI behavioral phenotypes are NF-κB dependent.
(A) Kaplan–Meier estimates of survival functions in TBI-treated NF-κB null mutants and sham-treated controls (n = 60/group) show that TBI significantly reduces life span (log-rank test, p < 0.001). (B) The effect of TBI on climbing behavior was tested in male NF-κB null mutants and sham-treated controls (n = 32/group for 7 consecutive days after TBI induction. There was no significant difference between both groups (n.s., t tests with Bonferroni correction. (C) TBI does not impair sleep in NF-κB null mutants, compared to sham-treated controls. TBI does not fragment sleep as average bout length (D) and bout number (E) are not changed. (F) Brief awakenings, a measure of sleep depth, are unchanged after TBI. (n = 57 controls, 49 in TBI group, n.s.; t tests with Bonferroni correction). Error bars indicate SEM. All figure-related data are located in S5 Data. NF-κB, nuclear factor kappa B; n.s., not significant; TBI, traumatic brain injury.