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Acute lymphoblastic leukemia

Serial evaluation of CD19 surface expression in pediatric B-cell malignancies following CD19-targeted therapy

Leukemia volume 34, pages 3064–3069 (2020)Cite this article

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CD19-targeting immunotherapies including blinatumomab, a (CD19/CD3) bispecific T-cell engaging antibody, and CD19-chimeric antigen receptor (CAR) T cells have been highly effective for B-cell acute lymphoblastic leukemia (ALL) [1, 2]. However, up to 50% of B-cell ALL patients relapse after CD19-targeted therapies, the majority with CD19-negative disease [3,4,5]. Given increasing utilization of these newly FDA-approved therapies in standard treatment paradigms, serial evaluations of CD19 surface expression following these therapies will be necessary to monitor for changes in leukemic phenotype.

As a referral center for therapies targeting CD22, an alternate B-cell antigen, we have a unique population of children and young adults with relapse or refractory disease to CD19 targeting [6,7,8]. Utilizing this cohort, we retrospectively analyzed serial CD19 surface expression to evaluate for dynamic changes in surface expression following immunotherapy.

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Fig. 1: Changes in CD19 Expression.

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Acknowledgements

We gratefully acknowledge the study participants and their families, referring medical care teams, the faculty and staff of the NIH Clinical Center who provide their expertise in the management of the study participants, and the data managers involved with this work. This work was supported in part by the Intramural Research Program, National Cancer Institute and NIH Clinical Center, National Institutes of Health. This research was made possible through the NIH Medical Research Scholars Program, a public–private partnership supported jointly by the NIH and contributions to the Foundation for the NIH from the Doris Duke Charitable Foundation (DDCF Grant #2014194), the American Association for Dental Research, the Colgate-Palmolive Company, Genentech, Elsevier, and other private donors. This work was supported in part by the Intramural Research Program of the National Institutes of Health, National Cancer Institute, Center for Cancer Research, and the Warren Grant Magnuson Clinical Center.

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Authors and Affiliations

  1. Pediatric Oncology Branch, Center for Cancer Research (CCR), National Cancer Institute (NCI), NIH, Bethesda, MD, USA

    Diane Libert, Haneen Shalabi, Bonnie Yates, Cindy Delbrook, Jack F. Shern, Terry J. Fry & Nirali N. Shah

  2. Laboratory of Pathology, CCR, NCI, NIH, Bethesda, MD, USA

    Constance M. Yuan, Pallavi Galera, Dalia Salem & Maryalice Stetler-Stevenson

  3. Oncogenomics Section, Genetics Branch, CCR, NCI, NIH, Bethesda, MD, USA

    Katherine E. Masih & Javed Khan

  4. Division of Human Immunology and Immunotherapy Initiative, Pediatric Hematology/Oncology, Children’s Hospital of Colorado, Aurora, CO, USA

    Terry J. Fry

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  1. Diane Libert
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Correspondence to Nirali N. Shah.

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Libert, D., Yuan, C.M., Masih, K.E. et al. Serial evaluation of CD19 surface expression in pediatric B-cell malignancies following CD19-targeted therapy. Leukemia 34, 3064–3069 (2020). https://doi.org/10.1038/s41375-020-0760-x

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