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Truncated and full-length TrkB receptors regulate distinct modes of dendritic growth

Nature Neuroscience volume 3, pages 342–349 (2000)Cite this article

Abstract

Neurotrophin regulation of neuronal morphology is complex and may involve differential action of alternative Trk receptor isoforms. We transfected ferret visual cortical slices with full-length and truncated TrkB receptors to examine their roles in regulating cortical dendrite development. These TrkB isoforms had differential effects on dendritic arborization: whereas full-length TrkB increased proximal dendritic branching, truncated TrkB promoted net elongation of distal dendrites. The morphological effects of each receptor isoform were distinct, yet their actions inhibited one another. Actions of the truncated TrkB receptor did not involve unmasking of endogenous TrkC signaling. These results suggest that TrkB receptors do not regulate dendritic growth per se but, rather, the mode of such growth.

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Figure 1: Cotransfection of TrkB isoforms and BFP into cortical pyramidal neurons using particle-mediated gene transfer.
Figure 2: Layer VI pyramidal neurons transfected with epitope-tagged TrkB isoforms.
Figure 3: T1 and full-length TrkB transfections induce outgrowth in distinct regions of the dendritic arbor.
Figure 4: Full-length TrkB increases dendritic branching whereas T1 induces elongation of pre-existing dendrites.
Figure 5: BDNF and NT-4/5 potentiate full-length TrkB's dendritic effects but not those of T1.
Figure 6: K252a blocks proximal dendritic outgrowth induced by exogenous and endogenous full-length TrkB receptor activation.
Figure 7: T1 induces distal dendritic growth by inhibiting full-length TrkB activation.
Figure 8: T1's effects on morphology are not mediated by TrkC.

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Acknowledgements

We thank D. Kryl and P. Barker for providing myc-tagged TrkB and TrkC plasmids, A. Haapasalo and E. Castren for providing FLAG-tagged TrkB, M. Bolton, N. Cant, D. Chikaraishi, L. Katz, J. McNamara, A. Pittman and N. Tang Sherwood for comments and advice and Regeneron Pharmaceuticals for providing BDNF and NT-4/5. This work was supported by grants from the NIH (NEI EY11553), the McKnight Endowment Fund for Neuroscience, the Ruth K. Broad Biomedical Research Foundation and the Duke Medical Scientist Training Program.

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  1. Department of Neurobiology, Duke University Medical Center, Box 3209, Durham, 27710, North Carolina, USA

    Talene A. Yacoubian & Donald C. Lo

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Correspondence to Donald C. Lo.

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Yacoubian, T., Lo, D. Truncated and full-length TrkB receptors regulate distinct modes of dendritic growth. Nat Neurosci 3, 342–349 (2000). https://doi.org/10.1038/73911

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