Abstract
Bone marrow cells infected with retroviral vectors carrying the bacterial neomycin resistance (neo) gene as a marker were used for long-term reconstitution of the haematopoietic system of irradiated mice. The neo gene is expressed in the myeloid and lymphoid lineages of these animals and an analysis of the sites of viral integration indicates that these lineages are derived from the same primitive multipotent cells.
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References
Till, J. E. & McCulloch, E. A. Radiat. Res. 14, 213–222 (1961).
Metcalf, D. Recent Results in Cancer Research (Springer, Berlin, 1977).
Johnson, G. R. & Metcalf, D. Proc. natn. Acad. Sci. U.S.A. 74, 3879–3882 (1977).
Iscove, N.N. in Haematopoietic Cell Differentiation (eds Golde, D. W., Cline, M. J., Metcalf, D. & Fox, C. F.) 37–52 (Academic, New York, 1978).
Suda, T., Suda, J. & Ogawa, M. Proc. natn. Acad. Sci. U.S.A. 80, 6689–6693 (1983).
Wu, A. M., Till, J. E., Siminovitch, L. & McCulloch, E. A. J. exp. Med. 127, 455–463 (1968).
Edwards, G. E., Miller, R. G. & Phillips, R. A. J. Immun. 105, 719–729 (1970).
Abramson, S., Miller, R. G. & Phillips, R. A. J. exp. Med. 145, 1567–1579 (1977).
Mintz, B., Anthony, K. & Litwin, S. Proc. natn. Acad. Sci. U.S.A. 81, 7835–7839 (1984).
Fialkow, P.J. J. cell. Physiol. Suppl. 1, 37–43 (1982).
Reiss, B., Sprengel, R., Will, H. & Schaller, H. Gene 30, 211–218 (1984).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
Paige, C.J. & Skarvall, H. J. immun. Meth. 52, 51–61 (1982).
Iscove, N. N. in Leukemia Dahlem Konferenzen (ed. Weissman, I. L.) (Springer, Berlin, in the press).
Williams, D. A., Lemischka, I. R., Nathan, D. G. & Mulligan, R. C. Nature 310, 476–480 (1984).
Miller, A. D., Eckner, R.J., Jolly, D.J., Friedmann, T. & Verma, I.M. Science 225, 630–632 (1984).
Hodgson, G. S. & Bradley, T. R. Nature 281, 381–382 (1979).
Joyner, A., Keller, G., Phillips, R. A. & Bernstein, A. Nature 305, 556–558 (1983).
Dick, J. E., Magli, M. C., Huszar, D., Phillips, R. A. & Bernstein, A. Cell 42, 71–79 (1985).
Iscove, N. N. in Methods for Serum-Free Cultures of Neuronal and Lymphoid Cells (Liss, New York, 1984).
Tambourin, P. E., Wendling, F., Gallien-Lartigue, O. & Huaulme, D. Biomedicine 19, 112–116 (1973).
Iscove, N. N., Keller, G. & Roitech, C. in Stem Cell Physiology (ed. Palek, J.) (Liss, New York, 1985).
Harrison, D. E. & Astle, C. M. J. exp. Med. 156, 1767–1779 (1982).
Mann, R., Mulligan, R. C. & Baltimore, D. Cell 33, 153–159 (1983).
Müller, R. & Müller, D. EMBO J. 3, 1121–1127 (1984).
Farrar, J. et al. J. Immun. 125, 2555–2558 (1980).
Nagao, K., Yokoro, K. & Aaronson, S. A. Science 212, 333–335 (1981).
Yung, Y., Eger, R., Tertian, G. & Moore, M. A. S. J. Immun. 121, 794–799 (1981).
Bloom, G. & Kelly, J. W. Histochemie 2, 48–57 (1960).
Dacie, J.V. & Lewis, S.N. Practical Hematology 5th edn (Churchill-Livingstone, Edinburgh, 1975).
Fazakas de St. Groth, S. & Scheidegger, D. J. immun. Meth. 35, 1–21 (1980).
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Keller, G., Paige, C., Gilboa, E. et al. Expression of a foreign gene in myeloid and lymphoid cells derived from multipotent haematopoietic precursors. Nature 318, 149–154 (1985). https://doi.org/10.1038/318149a0
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DOI: https://doi.org/10.1038/318149a0
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