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  • Genetics and Genomics
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Methylation status of oestrogen receptor-α gene promoter sequences in human ovarian epithelial cell lines

British Journal of Cancer volume 86, pages 282–284 (2002)Cite this article

Abstract

We have determined the methylation status of the CpG island of the oestrogen receptor α gene in seven human ovarian cell lines. Cell lines expressing oestrogen receptor α showed no evidence of hypermethylation. In three of four cell lines that produced no detectable oestrogen receptor α protein, hypermethylation was observed at the NotI site of the CpG island. These results indicate that aberrant hypermethylation may be responsible for a significant proportion of epithelial ovarian tumours in which oestrogen receptor α expression is lost.

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Acknowledgements

This work was supported by funding from Action Cancer, The Belfast City Hospital and the European Union European Social Fund. We are indebted to Dr J Herman, Johns Hopkins Oncology Center, for advice on MS–PCR and the provision of primer sequences.

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Author notes

  1. A M O'Doherty

    Present address: Department of Neurogenetics, Imperial College of Science, Technology & Medicine, School of Medicine, Norfolk Place, London, UK

  2. I Hickey: St. Mary's University College, Falls Road, Belfast, Northern Ireland, UK

Authors and Affiliations

  1. School of Biology and Biochemistry, The Queen's University of Belfast, Lisburn Road, Belfast, Northern Ireland, UK

    A M O'Doherty, J Nelson & I Hickey

  2. Department of Oncology, The Queen's University of Belfast, Lisburn Road, Belfast, Northern Ireland, UK

    S W Church & S E H Russell

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  1. A M O'Doherty
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  2. S W Church
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  3. S E H Russell
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  4. J Nelson
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  5. I Hickey
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Correspondence to I Hickey.

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O'Doherty, A., Church, S., Russell, S. et al. Methylation status of oestrogen receptor-α gene promoter sequences in human ovarian epithelial cell lines. Br J Cancer 86, 282–284 (2002). https://doi.org/10.1038/sj.bjc.6600028

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  • DOI: https://doi.org/10.1038/sj.bjc.6600028

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