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Production of medium chain length polyhydroxyalkanoates by fed-batch culture of Pseudomonas oleovorans

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Abstract

Pseudomonas oleovorans was cultivated to produce medium chain length polyhydroxyalkanoates (MCL-PHAs) from octanoic acid and ammonium nitrate as carbon and nitrogen source, respectively, by a pH-stat fed-batch culture technique. The octanoate in the culture broth was maintained below 4 g l−1 by feeding the mixture of octanoic acid and ammonium nitrate when the culture pH rose above 7.1. The final cell concentrations of 63, 55 and 9.5 g l−1, PHA contents of 62, 75 and 67% of dry cell wt, and productivities of 1, 0.63 and 0.16 g l−1 h−1 were obtained when the C/N ratios in the feed were 10, 20 and 100 g octanoic acid g−1 ammonium nitrate, respectively.

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  1. Department of Chemical Engineering, Chungbuk National University, Cheongju, Chungbuk, 361-763, Korea

    Beom Soo Kim

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Kim, B.S. Production of medium chain length polyhydroxyalkanoates by fed-batch culture of Pseudomonas oleovorans . Biotechnology Letters 24, 125–130 (2002). https://doi.org/10.1023/A:1013898504895

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  • DOI: https://doi.org/10.1023/A:1013898504895