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Production of poly(3-hydroxybutyrate) from whey by fed-batch culture of recombinant Escherichia coli in a pilot-scale fermenter

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Abstract

Production of poly(3-hydroxybutyrate) [P(3HB)] from wheyby fed-batch culture of recombinant Escherichia coli CGSC 4401 harboring a plasmid containing the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes was examined in a 30 l fermenter supplying air only. With lactose below 2 g l−1, cells grew to 12 g dry cell l−1 with 9% (w/w) P(3HB) content. Accumulation of P(3HB) could be triggered by increasing lactose to 20 g l−1. By employing this strategy, 51 g dry cell l−1 was obtained with a 70% (w/w) P(3HB) content after 26 h. The productivity was 1.35 g P(3HB) l−1 h−1. The same fermentation strategy was used in a 300 l fermenter, and 30 g dry cell l−1 with 67% (w/w) P(3HB) content was obtained in 20 h.

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Authors and Affiliations

  1. Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yusong-gu, Taejon, 305-701, Korea

    Si Jae Park, Jong Pil Park & Sang Yup Lee

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  1. Si Jae Park
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  2. Jong Pil Park
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  3. Sang Yup Lee
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Jae Park, S., Pil Park, J. & Yup Lee, S. Production of poly(3-hydroxybutyrate) from whey by fed-batch culture of recombinant Escherichia coli in a pilot-scale fermenter. Biotechnology Letters 24, 185–189 (2002). https://doi.org/10.1023/A:1014196906095

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  • DOI: https://doi.org/10.1023/A:1014196906095