Abstract
A viable mechanism for primer-dimer formation in polymerase chain reaction (PCR) process has been proposed based on experimental results. This mechanism results in a kinetic description of the primer-dimer formation process with the Taq DNA polymerase enzyme, the two primers and the dNTPs as the starting materials. The possible impact of this primer-dimer formation on the selectivity and yield of PCR process is discussed.
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Das, S., Mohapatra, S.C. & Hsu, J.T. Studies on primer-dimer formation in polymerase chain reaction (PCR). Biotechnology Techniques 13, 643–646 (1999). https://doi.org/10.1023/A:1008924615839
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DOI: https://doi.org/10.1023/A:1008924615839