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Production of poly(3-hydroxybutyrate) from whey by cell recycle fed-batch culture of recombinant Escherichia coli

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Abstract

A new fermentation strategy using cell recycle membrane system was developed for the efficient production of poly(3-hydroxybutyrate) (PHB) from whey by recombinant Escherichia coli strain CGSC 4401 harboring the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes. By cell recycle, fed-batch cultivation employing an external membrane module, the working volume of fermentation could be constantly maintained at 2.3 l. The final cell concentration, PHB concentration and PHB content of 194 g l−1, 168 g l−1 and 87%, respectively, were obtained in 36.5 h by the pH-stat cell recycle fed-batch culture using whey solution concentrated to contain 280 g lactose l−1 as a feeding solution, resulting in a high productivity of 4.6 g PHB l−1 h−1.

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Authors and Affiliations

  1. Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yusong-gu, Taejon, 305-701, Korea

    Woo Suk Ahn, Si Jae Park & Sang Yup Lee

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  1. Woo Suk Ahn
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  2. Si Jae Park
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  3. Sang Yup Lee
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Correspondence to Sang Yup Lee.

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Ahn, W.S., Park, S.J. & Lee, S.Y. Production of poly(3-hydroxybutyrate) from whey by cell recycle fed-batch culture of recombinant Escherichia coli. Biotechnology Letters 23, 235–240 (2001). https://doi.org/10.1023/A:1005633418161

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  • DOI: https://doi.org/10.1023/A:1005633418161