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Expression of microRNA-452 via adenoviral vector inhibits non-small cell lung cancer cells proliferation and metastasis

  • Original Article
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Tumor Biology

Abstract

The microRNA miR-452 has been shown to function as a tumor suppressor. However, the cellular mechanism and potential application of miR-452-mediated cancer suppression remain great unknown. This study aims to identify how miR-452 acts in regulating non-small cell lung cancer (NSCLC) proliferation and metastasis. Expression of miR-452 via adenoviral (Ad) vector inhibits the proliferation, invasion, and migration of NSCLC cells A549 or H460. Our data also shows that miR-452 down-regulates the expression of Bmi-1 as well as pro-survival or anti-apoptosis regulators Survivin, cIAP-1, and cIAP-2. By such gene interference, miR-452 modulates NSCLC cell epithelial–mesenchymal transition (EMT) and further disrupts their migration and invasion. Moreover, miR-452 blocks the activation of PI3K/AKT pathway, which is also required for EMT process. These data reveal that miR-452 treatment could be a novel target or strategy for NSCLC treatment.

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Acknowledgments

The authors thank Dr. Yu Cao in Medical College of Georgia, Georgia Regents University, for his helpful advices.

Author contributions

Conceived and designed the experiments: YZ JP. Performed the experiments: JP YW FF. Analyzed the data: QJ. Contributed reagents/materials/analysis tools: JP. Wrote the paper: YZ FF.

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Author notes

    Authors and Affiliations

    1. Department of Respiratory Diseases, The 463 Hospital of Chinese PLA, Shenyang, 110042, China

      Yongsheng Zhang, Jian Pang & Yang Wang

    2. Unit II, Department of Medical Oncology, The General Hospital of Chinese PLA, Beijing, 100853, China

      Lu Han

    3. Department of Pharmacy, General Hospital of Shenyang Military Command Area, Shenyang, 110016, China

      Fan Feng

    4. Center of Technical and Service, The 302nd Hospital of Chinese PLA, Beijing, 100039, China

      Qiyu Jiang

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    1. Yongsheng Zhang
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    2. Lu Han
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    5. Fan Feng
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    Correspondence to Yongsheng Zhang.

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    Yongsheng Zhang and Lu Han contributed equally to this work.

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    Zhang, Y., Han, L., Pang, J. et al. Expression of microRNA-452 via adenoviral vector inhibits non-small cell lung cancer cells proliferation and metastasis. Tumor Biol. 37, 8259–8270 (2016). https://doi.org/10.1007/s13277-015-4725-z

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    • DOI: https://doi.org/10.1007/s13277-015-4725-z

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