Abstract
The Bacillus subtilis natto fermented soy protein isolate (FSPI) exhibited concentration-dependent scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS+·) and hydroxyl (·OH) free-radicals. In addition, FSPI administration significantly increased superoxide dismutase activity in mice liver and serum by 20.2% and 86.2%, and suppressed the production of malondialdehyde by 51.3% and 35.1%, respectively, compared to high-fat control (HFC) group. Notably, the movement of mice treated with FSPI was livelier and more active, and its weight gain was significantly lower than that of both NC and HFC groups. The production and accumulation of perirenal fat was also significantly inhibited by FSPI, however, no significant difference in TG and TC levels were observed between FSPI and HFC groups. The results revealed the great potential of FSPI applying in the development of health food or sports food.
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Abbasi S, Gharaghani S, Benvidi A, Latif A (2018) Identifying the novel natural antioxidants by coupling different feature selection methods with nonlinear regressions and gas chromatography–mass spectroscopy. Microchem J 139:372–379. https://doi.org/10.1016/j.microc.2018.03.012
Boden G, Homko C, Barrero CA, Stein TP, Chen X, Cheung P, Fecchio C, Koller S, Merali S (2015) Excessive caloric intake acutely causes oxidative stress, GLUT4 carbonylation, and insulin resistance in healthy men. Sci Transl Med 304(7):1–9. https://doi.org/10.1021/jf011718g
Chung APYS, Sunil G, Srikumar C, Mohanambal M, Palanisamy UD (2018) Geraniin protects high-fat diet-induced oxidative stress in sprague dawley rats. Front Nutr 5:17–30. https://doi.org/10.3389/fnut.2018.00017
Fang Y, Yang S, Wu G (2002) Free radicals, antioxidants, and nutrition. Nutrition 18:872–879. https://doi.org/10.1016/S0899-9007(02)00916-4
Floegel A, Kim D, Chung S, Koo SI, Chun OK (2011) Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. J Food Compos Anal 24(7):1043–1048. https://doi.org/10.1016/j.jfca.2011.01.008
Gecer MK, Kan T, Gundogdu M, Ercisli S, Ilhan G, Sagbas HI (2020) Physicochemical characteristics of wild and cultivated apricots (Prunus armeniaca L.) from Aras valley in Turkey. Genet Resour Crop Evol 67:935–945. https://doi.org/10.1007/s10722-020-00893-9
Halliwell B, Gutteridge JMC, Aruoma OI (1987) The deoxyribose method: a simple ‘‘test tube’’ assay for determination of rate constants for reactions of hydroxyl radicals. Anal Biochem 165:215–219. https://doi.org/10.1016/0003-2697(87)90222-3
Iwai K, Nakaya N, Kawasaki Y, Matsue H (2002a) Antioxidative functions of Natto, a kind of fermented soybeans: effect on LDL oxidation and lipid metabolism in cholesterol-fed rats. J Agric Food Chem 50:3597–3601. https://doi.org/10.1021/jf0117199
Iwai K, Nakaya N, Kawasaki Y, Matsue H (2002b) The inhibitory effect of natto, a kind of fermented soybeans, on LDL oxidation in vitro. J Agric Food Chem 50:3592–3596. https://doi.org/10.1021/jf011718g
Kanazawa T, Tanaka M, Uemura T, Osanai T, Onodera K, Okubo K, Metoki H, Oike Y (1993) Anti-atherogenicity of soybean protein. Ann N Y Acad Sci 676:202–214. https://doi.org/10.1111/j.1749-6632.1993.tb38735.x
Li TP, Li SH, Du LJ, Wang N, Guo M, Zhang JW, Yan FW, Zhang HL (2010) Effects of haw pectic oligosaccharide on lipid metabolism and oxidative stress in experimental hyperlipidemia induced by a high-fat diet in mice. Food Chem 121:1010–1013. https://doi.org/10.1016/j.foodchem.2010.01.039
Li TP, Li SH, Dong YP, Zhu RG, Liu YH (2014a) Atioxidant activity of pentaoligosaccharide isolated from haw pectin suppresses triglyceride synthesis in high-fat fed mice. Food Chem 145:335–341. https://doi.org/10.1016/j.foodchem.2013.08.036
Li TP, Liu YH, Dong YP, Li SH, Zhu RG (2014b) Anti-fat deposition and antioxidant effects of haw pectic oligosaccharide in the liver of mice fed a high fat diet. CYTA J Food 12:27–31. https://doi.org/10.1080/19476337.2013.783625
Senica M, Stampar F, Mikulic-Petkovsek M (2019) Different extraction processes affect the metabolites in blue honeysuckle (Lonicera caerulea L. subsp. edulis) food products. Turk J Agric For 43:576–585
Shen YB, Zhang H, Cheng LL, Wang L, Qi XG (2016) In vitro and in vivo antioxidant activity of polyphenols extracted from black highland barley. Food Chem 194:1003–1012. https://doi.org/10.1016/10.1016/j.foodchem.2015.08.083
Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC (1985) Measurement of protein using bicinchoninic acid. Anal Biochem 150:76–85. https://doi.org/10.1016/0003-2697(85)90442-7
Stygar D, Skrzep-Poloczek B, Romuk E, Chełmecka E, Poloczek J, Sawczyn T, Maciarz J, Kukla M, Karcz KW, Jochem J (2019) The influence of high-fat, high-sugar diet and bariatric surgery on hsp70 and hsp90 plasma and liver concentrations in diet-induced obese rats. Cell Stress Chaperones 24(2):427–439. https://doi.org/10.1007/s12192-019-00976-2
Togo M, Konari N, Tsukamoto M, Kimoto R, Yamaguchi T, Takeda H, Kambayashiet I (2018) Effects of a high-fat diet on superoxide anion generation and membrane fluidity in liver mitochondria in rats. J Int Soc Sports Nutr 15:13. https://doi.org/10.1186/s12970-018-0217-z
Wu H, Wang H, Xu F, Chen JP, Duan LL, Zhang FJ (2019) Acute toxicity and genotoxicity evaluations of Nattokinase, a promising agent for cardiovascular diseases prevention. Regul Toxicol Pharmacol 103:205–209. https://doi.org/10.1016/j.yrtph.2019.02.006
Wang D, Wang LJ, Zhu FX, Zhu JY, Chen XD, Zou L, Saito M, Li LT (2018) In vitro and in vivo studies on the antioxidant activities of the aqueous extracts of Douchi (a traditional Chinese salt-fermented soybean food). Food Chem 107:1421–1428. https://doi.org/10.1016/j.foodchem.2007.09.072
Weng SJ, Yan DY, Gu LJ, Chen L, Xie ZJ, Wu ZY, Tang JH, Shen ZJ, Li H, Bai BL, Boodhun V, Yang L (2019) Combined treatment with vitamin K2 and PTH enhanced bone formation in ovariectomized rats and increased differentiation of osteoblast in vitro. Chem Biol Interact 300:101–110. https://doi.org/10.1016/j.cbi.2019.01.012
Yang YH, Wang YN, Sun J, Zhang JH, Guo HT, Shi YH, Cheng XG, Tang X, Le GW (2019) Dietary methionine restriction reduces hepatic steatosis and oxidative stress in high-fat-fed mice by promoting H2S production. Food Funct 10:61–77. https://doi.org/10.1039/C8FO01629A
Zia-Ul-Haq M, Ahmad S, Qayum M, Ercisli S (2013) Compositional studies and antioxidant potential of Albizia lebbeck (L.) Benth. Pods and seeds. Turk J Biol 37:25–32
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Zhang, X., Sun, X., Li, W. et al. In vitro and in vivo antioxidant activities of soy protein isolate fermented with Bacillus subtilis natto. J Food Sci Technol 58, 3199–3204 (2021). https://doi.org/10.1007/s13197-020-04823-6
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DOI: https://doi.org/10.1007/s13197-020-04823-6