Abstract
In this article, a low-cost light intensity-modulated biosensor for in situ growth monitoring of microbes is reported. Saccharomyces cerevisiae in YPD medium (yeast) and Escherichia coli in Luria Bertani medium (E. coli) are used in this study as the microbes. A matched wavelength of light source being used to interact with the microbes, and it has been gets absorption or scatter as the growth of the microbe increases. The system is realized by embedding and arranging all the required components as a single device. The obtained sensor response for yeast and Escherichia coli illustrates the linear temporal response of light intensity with a fitting factor of 0.99 within the limits, with ± 5% of error. This sensor key advantage is that the real-time microbe growth can be monitored without taking out the sample from the prepared culture. The designed biosensing system is simple and of low cost, and the obtained results are following the response of standard instruments. Thus, the proposed biosensor system is well suitable for the in situ study of growth rate monitoring applications of the microbes in medical and biotechnology.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
N. Paras, Prasad, Introduction to Biophotonics (Wiley, Hoboken, 2003).
C.R. Lowe, Overview of biosensor and bioarray technologies, in Part one: handbook of biosensors and biochips. ed. by R.S. Marks, D.C. Cullen, I. Karube, C.R. Lowe and H.H. Weetall (2008)
J.H. Luong, K.B. Male, J.D. Glennon, Biosensor technology: technology push versus market pull. Biotechnol. Adv. 26(5), 492–500 (2008)
D.J. Ecker, R. Sampath, C. Massire, L.B. Blyn, T.A. Hall, M.W. Eshoo, S.A. Hofstadler, Ibis T5000: a universal biosensor approach for microbiology. Nat. Rev. Microbiol. 6(7), 553–558 (2008)
K. Kahn, K.W. Plaxco, Principles of biomolecular recognition, in Recognition receptors in biosensors. ed. by M. Zourob (Springer, New York, 2010), pp. 3–45
B.D. Malhotra, A. Turner (eds.), Advances in Biosensors: Perspectives in Biosensors, vol. 5 (Elsevier, Amsterdam, 2003).
V. Perumal, U. Hashim, Advances in biosensors: Principle, architecture and applications. J. Appl. Biomed. 12(1), 1–15 (2014)
I.K. Cigić, H. Prosen, An overview of conventional and emerging analytical methods for the determination of mycotoxins. Int. J. Mol. Sci. 10(1), 62–115 (2009)
N. Ke, X. Wang, X. Xu, Y.A Abassi, The xCELLigence system for real-time and label-free monitoring of cell viability. Mammalian Cell Viability: Methods and Protocols, p. 33–43 (2011)
A.C.R. Grayson, R.S. Shawgo, A.M. Johnson, N.T. Flynn, Y. Li, M.J. Cima, R. Langer, A BioMEMS review: MEMS technology for physiologically integrated devices. Proc. IEEE 92(1), 6–21 (2004)
S. Tsoka, A. Gill, J.L. Brookman, M. Hoare, Rapid monitoring of virus-like particles using an optical biosensor: a feasibility study. J. Biotechnol. 63(2), 147–153 (1998)
C. Védrine, J.C. Leclerc, C. Durrieu, C. Tran-Minh, Optical whole-cell biosensor using Chlorella vulgaris designed for monitoring herbicides. Biosens. Bioelectron. 18(4), 457–463 (2003)
D. Bhatta, E. Stadden, E. Hashem, I.J.G. Sparrow, G.D. Emmerson, Multi-purpose optical biosensors for real-time detection of bacteria, viruses and toxins. Sens Actuators B: Chem 149(1), 233–238 (2010)
N.Z. Md Muslim, M. Ahmad, L.Y. Heng, B. Saad, Optical biosensor test strip for the screening and direct determination of L-glutamate in food samples. Sens. Actuators B: Chem. 161, 493–497 (2012)
L.A. Nafie, T.B. Freedman, Biological and pharmaceutical applications of vibrational optical activity, in Infrared and raman spectroscopy of biological materials. ed. by H.U. Gremlich, B. Yan (Marcel Dekker, New York, 2001), pp. 15–54
O.W. Richards, The growth of the yeast Saccharomyces cerevisiae: the growth curve, its mathematical analysis and the effects of temparature on the yeast growth. Ann. Bot. 42(1), 271–283 (1928)
S. Hye-Kyoung, L. Dae-Ho, L. Mi-Hwa, K. Jong-Baeg, S. Jeon-Soo, K. Yong-Jun, MEMS based capacitive biosensor for real time detection of bacterial growth. J. Sensor Sci. Technol. 17(3), 195–202 (2008)
R. Shantilatha, S. Varma, C.K. Mitra, Designing a simple biosensor, in Advances in biosensors: perspectives in biosensor. ed. by B.D. Malhotra, A.P.F. Turner (JAI Press, Stamford, 2003), pp. 1–36
Y.W Kim, S. Subramanian, Biofilm Monitoring and Treatment. MEMS Sensors and Actuators Laboratory, A.J. Clark School of Engineering at the University of Maryland (UMD)
Y.W. Kim, S.E. Sardari, A.A. Iliadis, R. Ghodssi, A Bacterial Biofilm Surface Acoustic Wave Sensor for Real Time Biofilm Growth Monitoring. IEEE Sensors 2010, Waikoloa, HI, November 1–4 (2010) p. 1568–1571
P. Kishore, P.B. Ravindra, V. Rama Devi, T. Maunika, P. Soujanya, P.V.N. Kishore, D. Dinakar, Laser intensity modulated real time monitoring cell growth sensor for bioprocess applications, SPIE Photonics Europe held on 3–7 April 2016 in Brussels, Belgium, Proc. SPIE 9899, Optical Sensing and Detection IV, 98992A (2016)
Author information
Authors and Affiliations
Corresponding author
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Kishore, P., Dinakar, D., Babu, P.R. et al. Laser light intensity-modulated cell growth rate monitoring biosensor. J Opt 50, 28–34 (2021). https://doi.org/10.1007/s12596-020-00673-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12596-020-00673-9