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Production of polyhydroxyalkanoates by Azotobacter vinelandii UWD using swine wastewater: Effect of supplementing glucose, yeast extract, and inorganic salts

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Abstract

This study examined the effect of adding glucose, yeast extract, and inorganic salts to swine wastewater (SWW) in a batch culture on the production of a biodegradable plastic, polyhydroxyalkanoate (PHA). A bacterial strain, Azotobacter vinelandii UWD, was used to produce PHA without limiting the non-carbon nutrients. The addition of glucose (30 g/L) to the SWW medium increased the level of cell growth (4.4∼7.0 times) and PHA production (3.8∼8.5 times) depending upon the dilution of SWW. A 50% dilution of SWW was found to be optimal considering the dry cell weight (9.40 g/L), PHA content (58 wt%), and hydroxyvalerate (HV) mol fraction in the PHA (4.3 mol%). A 75% SWW medium was more advantageous for producing PHA with a higher HV fraction (7.1 mol%) at the expense of losing 22% of PHA production. The undiluted SWW medium produced less than one third of the PHA compared with the 50% SWW medium, but the HV fraction was the highest (10.8 mol%). Regarding the effect of the glucose concentration, at 20 g/L glucose, the dry cell weight and level of PHA production increased to 9.34 g/L (0.63 g PHA/g dry cell weight) and 5.90 g/L, respectively. At 50 g/L glucose, there was no significant increase in PHA production. For the glucose-supplemented (30 g/L) 50% SWW medium, the addition of a nitrogen source (1 g/L of yeast extract) did not increase the level of cell growth or PHA production because the C:N ratio (23:1) was already close to the optimal value (22:1). Better aeration increased the productivity of PHA. External nitrogen supplements (1 g/L of yeast extract) and other essential mineral salts was not necessary for bacterial growth because they were contained in the SWW. These results suggest that SWW is an excellent feedstock for producing larger amounts of the value-added material, PHA, if it is combined with carbohydrate-rich organic waste.

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Authors and Affiliations

  1. Department of Chemical and Environmental Engineering, Soongsil University, Seoul, 156-743, Korea

    Hee Wook Ryu

  2. Department of Environmental Science and Engineering, Ewha Womans University, Seoul, 120-750, Korea

    Kyung Suk Cho

  3. University of Minnesota, 1390 Eckles Avenue, St. Paul, MN, 55108, USA

    Philip R. Goodrich

  4. Industrial Liaison Research Institute, Green Energy Center, and College of Environment and Applied Chemistry, Kyung Hee University, Yong-in, 446-701, Korea

    Chang-Ho Park

Authors
  1. Hee Wook Ryu
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  2. Kyung Suk Cho
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  3. Philip R. Goodrich
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  4. Chang-Ho Park
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Correspondence to Chang-Ho Park.

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Ryu, H.W., Cho, K.S., Goodrich, P.R. et al. Production of polyhydroxyalkanoates by Azotobacter vinelandii UWD using swine wastewater: Effect of supplementing glucose, yeast extract, and inorganic salts. Biotechnol Bioproc E 13, 651–658 (2008). https://doi.org/10.1007/s12257-008-0072-x

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  • DOI: https://doi.org/10.1007/s12257-008-0072-x

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