[go: up one dir, main page]
More Web Proxy on the site http://driver.im/ Skip to main content
Log in

Nano-Au/cMWCNT Modified speB Gene Specific Amperometric Sensor for Rapidly Detecting Streptococcus pyogenes causing Rheumatic Heart Disease

  • Short Communication
  • Published:
Indian Journal of Microbiology Aims and scope Submit manuscript

Abstract

A specific 5′ NH2 labeled DNA probe of speB gene was immobilized onto the gold nanoparticles/carboxylated multi walled carbon nanotubes (Nano-Au/cMWCNT) screen printed electrode using EDC/NHS cross linking chemistry. This was followed by hybridization with 0.5–50 ng/6 µl of single stranded genomic DNA Streptococcus pyogenes infected patient throat swab samples. Electrochemical amperometric assay was deciphered by using cyclic voltammetry (CV) with methylene blue a redox indicator. The sensor had a sensitivity of 104.7 µA cm−2 ng−1 using CV with a R2 of 0.907 and 0.01 ng/6 µl as the limit of detection (LOD). The modified electrode surface morphology was characterized using scanning electron microscopy. The stability of the electrode was seen at 4 °C for 180 days having 6% loss in the initial current. The sensor is speB gene specific and can detect the pathogen within 30 min.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
£29.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (United Kingdom)

Instant access to the full article PDF.

Scheme 1
Fig. 1
Fig. 2

References

  1. Singh S, Kaushal A, Khare S, Kumar A (2014) mga genosensor for early detection of human rheumatic heart disease. Appl Biochem Biotechnol 173:228–238. doi:10.1007/s12010-014-0836-z

    Article  CAS  PubMed  Google Scholar 

  2. Guilherme L, Ko¨hler KF, Postol E, Kalil J (2011) Genes, autoimmunity and pathogenesis of rheumatic heart disease. Ann Pediatr Cardiol 4:13–21. doi:10.4103/0974-2069.79617

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Guilherme L, Fae´ K, Oshiro SE, Kalil J (2005) Molecular pathogenesis of rheumatic fever and rheumatic heart disease. Expert Rev Mol Med 7:1–15. doi:10.1017/S146239940501015X

    Article  PubMed  Google Scholar 

  4. Kalia VC, Kumar R, Kumar P, Koul S (2016) A genome-wide profiling strategy as an aid for searching unique identification biomarkers for Streptococcus. Indian J Microbiol 56:46–58. doi:10.1007/s12088-015-0561-5

    Article  CAS  PubMed  Google Scholar 

  5. Koul S, Kumar P, Kalia VC (2015) A unique genome wide approach to search novel markers for rapid identification of bacterial pathogens. J Mol Genet Med 9:194. doi:10.4172/1747-0862.1000194

    Article  Google Scholar 

  6. Singh S, Kaushal A, Gupta S, Kumar A (2016) Gene specific impedimetric bacterial DNA sensor for rheumatic heart disease. Indian J Microbiol. doi:10.1007/s12088-016-0620-6

    Google Scholar 

  7. Collin M, Olsén A (2001) Effect of SpeB and EndoS from Streptococcus pyogenes on human immunoglobulins. Infect Immun 69:7187–7189. doi:10.1128/IAI.69.11.7187-7189.2001

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Parida MM, Sannarangaiah S, Dash PK, Rao PVL, Morita K (2008) Loop mediated isothermal amplification (LAMP): a new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases. Rev Med Virol 18:407–421. doi:10.1002/rmv.593

    Article  CAS  PubMed  Google Scholar 

  9. Muldrew KL (2009) Molecular diagnostics of infectious diseases. Curr Opin Pediatr 21:102–111. doi:10.1097/MOP.0b013e328320d87e

    Article  PubMed  Google Scholar 

  10. Kumar A, Bhatnagar A, Gupta S, Khare S, Suman S (2011) sof gene as a specific genetic marker for detection of Streptococcus pyogenes causing pharyngitis and rheumatic heart disease. Cell Mol Biol 57:26–30. doi:10.1170/T898

    CAS  PubMed  Google Scholar 

  11. Kaushal A, Kumar D, Khare S, Kumar A (2012) speB gene as a specific genetic marker for early detection of rheumatic heart disease in human. Cell Mol Biol (noisy-le-grand) 58:50–54. doi:10.1170/T920

    CAS  Google Scholar 

  12. Singh S, Kaushal A, Khare S, Kumar A (2014) Gold-mercaptopropionic acid polyethylenimine composite based DNA sensor for early detection of rheumatic heart disease. Analyst 139:3600–3606. doi:10.1039/C4AN00324A

    Article  CAS  PubMed  Google Scholar 

  13. Sheehan J, Cruickshank P, Boshart G (1961) Notes-a convenient synthesis of water-soluble carbodiimide. J Org Chem 26:2525–2528. doi:10.1021/jo01351a600

    Article  CAS  Google Scholar 

Download references

Acknowledgements

Dr. Shashi Khare, National Center for Disease Control (NCDC), Delhi and Prof. N.N. Mathur, ENT Department, Safdarjung Hospital are highly acknowledged for their constant support, providing facility and patient samples.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Dinesh Kumar.

Ethics declarations

Conflict of interest

Authors declare no conflict of interest.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 38 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kaushal, A., Singh, S., Kumar, A. et al. Nano-Au/cMWCNT Modified speB Gene Specific Amperometric Sensor for Rapidly Detecting Streptococcus pyogenes causing Rheumatic Heart Disease. Indian J Microbiol 57, 121–124 (2017). https://doi.org/10.1007/s12088-016-0636-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12088-016-0636-y

Keywords

Navigation