Abstract
A specific 5′ NH2 labeled DNA probe of speB gene was immobilized onto the gold nanoparticles/carboxylated multi walled carbon nanotubes (Nano-Au/cMWCNT) screen printed electrode using EDC/NHS cross linking chemistry. This was followed by hybridization with 0.5–50 ng/6 µl of single stranded genomic DNA Streptococcus pyogenes infected patient throat swab samples. Electrochemical amperometric assay was deciphered by using cyclic voltammetry (CV) with methylene blue a redox indicator. The sensor had a sensitivity of 104.7 µA cm−2 ng−1 using CV with a R2 of 0.907 and 0.01 ng/6 µl as the limit of detection (LOD). The modified electrode surface morphology was characterized using scanning electron microscopy. The stability of the electrode was seen at 4 °C for 180 days having 6% loss in the initial current. The sensor is speB gene specific and can detect the pathogen within 30 min.
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Acknowledgements
Dr. Shashi Khare, National Center for Disease Control (NCDC), Delhi and Prof. N.N. Mathur, ENT Department, Safdarjung Hospital are highly acknowledged for their constant support, providing facility and patient samples.
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Kaushal, A., Singh, S., Kumar, A. et al. Nano-Au/cMWCNT Modified speB Gene Specific Amperometric Sensor for Rapidly Detecting Streptococcus pyogenes causing Rheumatic Heart Disease. Indian J Microbiol 57, 121–124 (2017). https://doi.org/10.1007/s12088-016-0636-y
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DOI: https://doi.org/10.1007/s12088-016-0636-y