Abstract
Ischemic stroke is considered to be a complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. MicroRNAs participated in various physiopathological processes; common single-nucleotide polymorphisms (SNPs) in pre-miRNAs have been shown to be associated with susceptibility to several human diseases. We evaluated the associations of the hsa-mir-196-a2/rs11614913 T/C, hsa-mir-146a/rs2910164 C/G, and hsa-mir-499/rs3746444 A/G polymorphisms in pre-miRNAs with the risk of ischemic stroke in a Chinese population. The three polymorphisms were identified in 296 ischemic stroke patients and 391 healthy controls using polymerase chain reaction–restriction fragment length polymorphism. The frequency of the allele G of hsa-mir-499/rs3746444 A/G showed significant association with ischemic stroke when compared with controls (OR = 1.509, 95%CI = 1.151–1.978, P = 0.003). Increased ischemic stroke risks were associated with rs3746444 A/G genotypes in different genetic model (homozygote comparison: P = 0.045, OR = 2.084, 95%CI = 1.019–4.262; heterozygote comparison: P = 0.024, OR = 1.489, 95%CI = 1.063–2.087; dominant genetic model: P = 0.007, OR = 1.563, 95%CI = 1.135–2.153). Similar results were obtained by adjusted fully risk factors. However, we failed to find any association between the alleles and genotypes of rs2910164 C/G and rs11614913 T/C SNPs and ischemic stroke, respectively (p > 0.05). The present study provided evidence that hsa-mir-499/rs3746444 A/G polymorphism might be associated with a significantly increased risk of ischemic stroke in a Chinese population, indicating that the common genetic polymorphism in pre-microRNAs contributed to the pathogenesis of ischemic stroke.
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Acknowledgments
This Project was Supported by Scientific Research Fund of Sichuan Provincial Education Department (grant number: 10ZA079 and 2006B029) and Scientific Research Fund of Sichuan Provincial Health Department (grant number: 090136).
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Liu, Y., Ma, Y., Zhang, B. et al. Genetic Polymorphisms in Pre-microRNAs and Risk of Ischemic Stroke in a Chinese Population. J Mol Neurosci 52, 473–480 (2014). https://doi.org/10.1007/s12031-013-0152-z
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DOI: https://doi.org/10.1007/s12031-013-0152-z