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Effects of Selenium-Chitosan on Blood Selenium Concentration, Antioxidation Status, and Cellular and Humoral Immunity in Mice

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Abstract

One hundred and eighty Kunming mice were allotted to three groups in a randomized complete block design, including two treatments and one control. Mice in group 1 were fed a basal diet as control, while mice in groups 2 and 3 were fed the basal diet supplemented with 0.2 mg/kg selenium as sodium selenite (SS) or selenium-chitosan (SC), respectively. On day 28 of the experiment, blood selenium concentration, glutathione peroxidase (GPx) activity, plasma superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, and Con A-induced splenocyte proliferation were determined, and plasma interleukin-2 (IL-2) and interferon-γ (IFN-γ) concentrations, splenic plaque-forming cell (PFC) responses, serum hemolysis level (HC50), and delayed-type hypersensitivity (DTH) responses were determined on day 15 of the experiment. The results showed that blood selenium concentration, GPx activity, splenic PFC response, and plasma IL-2 and IFN-γ concentrations in SC group were higher than those in the control and SS groups (P < 0.01 or P < 0.05), respectively. Plasma SOD activity, Serum hemolysis level, DTH responses, and Con A-induced splenocyte proliferation in SC group were higher than those in control (P < 0.01 or P < 0.05). Plasma SOD activity, serum hemolysis level, DTH responses, and Con A-induced splenocyte proliferation in SC group were also higher than those in SS group, while there was no significant difference between SC and SS groups (P > 0.05). Plasma MDA content in SC group was lower than those in the control and SS groups (P < 0.01 or P < 0.05). It is concluded that SC supplement can increase blood selenium concentration, antioxidation status, and cellular and humoral immunity, and SC has better biological activity than SS in mice.

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (grant numbers 31101867 and 31001093), Development of Science and Technology Foundation of Higher Educational Institutions of Tianjin (grant number 20110620), and Program of Veterinary Biotechnology Outstanding Scientific Research Innovation Team of Tianjin (grant number TD12-5019).

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Authors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China

    Shunyi Qin, Baoxia Huang, Jifei Ma, Xin Wang, Jianbing Zhang & Liuan Li

  2. College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, 266109, Shangdong Province, China

    Fu Chen

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  1. Shunyi Qin
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Correspondence to Fu Chen.

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Qin, S., Huang, B., Ma, J. et al. Effects of Selenium-Chitosan on Blood Selenium Concentration, Antioxidation Status, and Cellular and Humoral Immunity in Mice. Biol Trace Elem Res 165, 145–152 (2015). https://doi.org/10.1007/s12011-015-0243-5

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