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Production of Thermostable Lipase by Thermomyces lanuginosus on Solid-State Fermentation: Selective Hydrolysis of Sardine Oil

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Abstract

A naturally immobilized biocatalyst with lipase activity was produced by Thermomyces lanuginosus on solid-state fermentation with perlite as inert support. Maxima lipase activities (22 and 120 U/g of dry matter, using p-nitrophenyl octanoate and trioctanoine, respectively, as substrates) were obtained after 72 h of solid culture, remaining nearly constant up to 120 h. Maxima lipase activity was found at 60 to 85 °C and pH 10. The biocatalyst was stable at 60 °C for at least 4 h of incubation and a pH from 7 to 10. Energy values of activation and deactivation of lipase were of 26 and 6.7 kJ/mol, respectively. The biocatalyst shows high selectivity for the release of the omega-3 polyunsaturated fatty acids, eicosapentaenoic (EPA) and docosahexaenoic acids (DHA), during the hydrolysis of sardine oil. The EPA/DHA ratio (16:6) released by this biocatalyst was superior to that obtained with the commercial preparations of T. lanuginosus.

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Acknowledgments

The authors acknowledge financial support (Project 154004) from The National Council for Science and Technology (CONACyT), and Nayeli Ávila acknowledges the scholarship number 203453. The authors gratefully acknowledge Dr. Gloria Fernández-Lorente for her support and technical assistance during the application of biocatalysts in the hydrolysis of sardine oil at the Research Institute of Food Science (CIAL), UAM, Madrid (Spain).

Author information

Author notes

  1. Ernesto Favela-Torres

    Present address: Departamento de Biotecnología, Universidad Autónoma Metropolitana—Unidad Iztapalapa, Av. San Rafael Atlixco 186, Col. Vicentina, Iztapalapa, Mexico, 09340, D.F., Mexico

Authors and Affiliations

  1. Universidad Autónoma Metropolitana—Unidad Iztapalapa, Av. San Rafael Atlixco 186, Col. Vicentina, Iztapalapa, Mexico, 09340, D.F., Mexico

    Nayeli Ávila-Cisneros, Susana Velasco-Lozano, Sergio Huerta-Ochoa & Ernesto Favela-Torres

  2. Departamento de Química, Universidad de Guadalajara, Guadalajara, Mexico

    Jesús Córdova-López

  3. Facultad de Química, Departamento de Alimentos y Biotecnología, Universidad Nacional Autónoma de México, Ciudad Universitaria, Mexico, D.F., 04510, Mexico

    Miquel Gimeno

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  1. Nayeli Ávila-Cisneros
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Correspondence to Ernesto Favela-Torres.

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Ávila-Cisneros, N., Velasco-Lozano, S., Huerta-Ochoa, S. et al. Production of Thermostable Lipase by Thermomyces lanuginosus on Solid-State Fermentation: Selective Hydrolysis of Sardine Oil. Appl Biochem Biotechnol 174, 1859–1872 (2014). https://doi.org/10.1007/s12010-014-1159-9

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  • DOI: https://doi.org/10.1007/s12010-014-1159-9

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