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Identification and Structure Elucidation of a Novel Antifungal Compound Produced by Pseudomonas aeruginosa PGPR2 Against Macrophomina phaseolina

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Abstract

Pseudomonas aeruginosa PGPR2 was found to protect mungbean plants from charcoal rot disease caused by Macrophomina phaseolina. Secondary metabolites from the culture supernatant of P. aeruginosa PGPR2 were extracted with ethyl acetate and the antifungal compound was purified by preparative HPLC using reverse phase chromatography. The purified compound showed antifungal activity against M. phaseolina and other phytopathogenic fungi (Fusarium sp., Rhizoctonia sp. Alternaria sp., and Aspergillus sp.). The structure of the purified compound was determined using 1H, 13C, 2D NMR spectra and liquid chromatography-mass spectrometry (LC-MS). Spectral data suggest that the antifungal compound is 3,4-dihydroxy-N-methyl-4-(4-oxochroman-2-yl)butanamide, with the chemical formula C14H17NO5 and a molecular mass of 279. Though chemically synthesized chromanone derivatives have been shown to have antifungal activity, we report for the first time, the microbial production of a chromanone derivative with antifungal activity. This ability of P. aeruginosa PGPR2 makes it a suitable strain for biocontrol.

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Acknowledgements

The work was financially supported by the Indian Council for Agricultural Research (NBAIM/AMAAS/2007-2012/MG (5)/PG/BG/3), India. The central facilities from CAS, CEGS, UGC-NRCBS and DBT- IPLS at MKU are gratefully acknowledged.

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Authors and Affiliations

  1. Department of Genetics, Centre for Excellence in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University, Madurai, 625 021, India

    Devaraj Illakkiam, Paramasivan Ponraj, Manoharan Shankar, Jeyaprakash Rajendhran & Paramasamy Gunasekaran

  2. Department of Organic Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, 625 021, India

    Shanmugam Muthusubramanian

Authors
  1. Devaraj Illakkiam
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  2. Paramasivan Ponraj
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  3. Manoharan Shankar
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  4. Shanmugam Muthusubramanian
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  5. Jeyaprakash Rajendhran
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  6. Paramasamy Gunasekaran
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Correspondence to Paramasamy Gunasekaran.

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Illakkiam, D., Ponraj, P., Shankar, M. et al. Identification and Structure Elucidation of a Novel Antifungal Compound Produced by Pseudomonas aeruginosa PGPR2 Against Macrophomina phaseolina . Appl Biochem Biotechnol 171, 2176–2185 (2013). https://doi.org/10.1007/s12010-013-0469-7

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  • DOI: https://doi.org/10.1007/s12010-013-0469-7

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