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Biochemical responses to drought stress in mulberry (Morus alba L.): evaluation of proline, glycine betaine and abscisic acid accumulation in five cultivars

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Abstract

Five popularly grown mulberry cultivars (K-2, MR-2, TR-10, BC2-59 and S-13) were subjected to drought stress by withholding irrigation, to obtain leaf water potentials (Ψw) ranging from −0.75, −1.50 and −2.25 MPa. Accumulation of proline, glycine betaine and abscisic acid (ABA) were quantified in control and water stressed mulberry leaves. The activities of enzymes involved in proline accumulation including glutamate dehydrogenase (EC1.4.1.2-4), pyrroline-5-carboxylate synthetase (EC 1.2.1.41), pyrroline-5-carboxylate reductase (EC1.5.1.2), ornithine transaminase (EC 2.6.1.13) were significantly enhanced in the leaves of all the cultivars with decreasing leaf water potentials, while the activities of proline dehydrogenase (EC 1.5.1.2) were reduced with progressive increase in water stress. Accumulation of proline, glycine betaine and abscisic acid was relatively higher in S-13 and BC2-59 compared to K-2, MR-2 and TR-10 under water deficit conditions. Our results demonstrate that S-13 and BC2-59 have superior osmoprotectant mechanisms under water-limited growth regimes.

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Acknowledgments

CSIR fellowship to K.V. Chaitanya is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Plant Biology and Biotechnology, School of Life Sciences, University of Hyderabad, Hyderabad, 500046, India

    Kolluru Viswanatha Chaitanya, Girish Kumar Rasineni & Attipalli Ramachandra Reddy

Authors
  1. Kolluru Viswanatha Chaitanya
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  2. Girish Kumar Rasineni
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  3. Attipalli Ramachandra Reddy
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Correspondence to Attipalli Ramachandra Reddy.

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Communicated by W. Filek.

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Chaitanya, K.V., Rasineni, G.K. & Reddy, A.R. Biochemical responses to drought stress in mulberry (Morus alba L.): evaluation of proline, glycine betaine and abscisic acid accumulation in five cultivars. Acta Physiol Plant 31, 437–443 (2009). https://doi.org/10.1007/s11738-008-0251-6

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  • DOI: https://doi.org/10.1007/s11738-008-0251-6

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