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Molecular characterization, morphology, and pathogenicity of Alternaria panax from araliaceous plants in Korea

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Abstract

Alternaria blight on araliaceous plants is a common disease caused by Alternaria panax Whetzel and occurs worldwide. Genetic diversity among 58 isolates of A. panax from different araliaceous hosts in Korea was determined by amplified fragment length polymorphism (AFLP) analysis. Selected isolates from genetic groups (determined by AFLP analysis) were examined based on the results of phylogenetic analyses of eight genes (Alt a1, BT1, BT2, EF-1α, gpd, H3, ITS, and RPB2), morphological characteristics, and pathogenicity tests. Isolates were divided into two distinct genetic groups (A and B) by AFLP analysis and based on the results of sequence analyses of the BT1, BT2, gpd, and RPB2 genes. Isolates from ginseng plants (Panax ginseng and P. quinquefolius) fell into group B, whereas isolates from the other araliaceous plants clustered in group A. Morphologically, although some overlap was observed among isolates in the two groups, isolates in group B had longer and narrower conidia, distinct sporulation patterns at 15 °C, and did not secrete pigment into PDA media. Pathogenicity tests revealed that isolates in groups A and B only induced severe disease symptoms on leaves of their hosts, such as isolates in group A on Aralia elata, Aralia continentalis, and in group B on Panax ginseng. The results indicate that the two genetic groups of Alternaria from araliaceous plants should be considered as two different species, and we propose that group A is a candidate of new Alternaria species distinguished from A. panax.

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References

  • Andersen B, Thrane U (1996) Differentiation of Alternaria infectoria and Alternaria alternata based on morphology, metabolite profiles, and cultural characteristics. Can J Microbiol 42:685–689

    Article  CAS  Google Scholar 

  • Andersen B, KrØger E, Roberts RG (2001) Chemical and morphological segregation of Alternaria alternata, A. gaisen and A. longipes species-groups. Mycol Res 105:291–299

    Article  CAS  Google Scholar 

  • Andersen B, KrØger E, Roberts RG (2002) Chemical and morphological segregation of Alternaria arboresens, A. infectoria and A. tenuissima species-groups. Mycol Res 106:170–182

    Article  CAS  Google Scholar 

  • Andersen B, Dongo A, Pryor BM (2008) Secondary metabolite profiling of Alternaria dauci, A. porri, A. solani, and A. tomatophila. Mycol Res 112:241–250

    Article  PubMed  CAS  Google Scholar 

  • Andersen B, Sorensen JL, Nielsen KF, Gerrits van den Ende B, de Hoog GS (2009) A polyphasic approach to the taxonomy of the Alternaria infectoria species-group. Fungal Genet Biol 46:642–656

    Article  PubMed  CAS  Google Scholar 

  • Aradhya MK, Chan HM, Parfitt DE (2001) Genetic variability in the pistachio late blight fungus, Alternaria alternata. Mycol Res 105:300–306

    Article  CAS  Google Scholar 

  • Atilano RA (1983) Alternaria leaf spot of Schefflera arboricola. Plant Dis 67:64–66

    Article  Google Scholar 

  • Aveskamp MM, Verkley GJM, de Gruyter J, Murace MA, Perelló A, Woudenberg JHC, Groenewald JZ, Crous PW (2009) DNA phylogeny reveals polyphyly of Phoma section Peyronellaea and multiple taxonomic novelties. Mycologia 101:363–382

    Article  PubMed  CAS  Google Scholar 

  • Barnes I, Crous PW, Wingfield BD, Wingfield MJ (2004) Multigene phylogenies reveal that red band needle blight of Pinus is caused by two distinct species of Dothistroma, D. septosporum and D. pini. Stud Mycol 50:551–565

    Google Scholar 

  • Berbee ML, Pirseyedi M, Hubbard S (1999) Cochliobolus phylogenetics and the origin of known, highly virulent pathogens, inferred from ITS and glyceraldehydes-3-phosphate dehydrogenase gene sequences. Mycologia 91:964–977

    Article  CAS  Google Scholar 

  • Bock CH, Thrall PH, Brubaker CL, Burdon JJ (2002) Detection of genetic variation in Alternaria brassicicola using AFLP fingerprinting. Mycol Res 106:428–434

    Article  CAS  Google Scholar 

  • Bruns TD, Vilgalys R, Barns SM, Gonzales D, Hibbet DS, Lane DJ, Simon L, Stickel S, Szaro TM, Weisberg WG, Sogin ML (1992) Evolutionary relationships within the fungi: analyses of nuclear small subunit rRNA sequences. Mol Phylogenet Evol 1:231–241

    Article  PubMed  CAS  Google Scholar 

  • Camara MPS, Palm ME, van Berkum P, O’Neill NR (2002) Molecular phylogeny of Leptosphaeria and Phaeosphaeria. Mycologia 94:630–640

    Article  PubMed  CAS  Google Scholar 

  • Carbone I, Anderson JB, Kohn LM (1999) Patterns of descent in clonal lineages and their multilocus finger prints are resolved with combined gene genealogies. Evolution 53:11–21

    Article  CAS  Google Scholar 

  • Chen YJ, Wang Y, Feng GQ, Liu YZ (2005) Biological characteristics of Alternaria panax Whetzel isolated from Panax notoginseng. ACTA Phytopathologica Sinica 35:267–269

    Google Scholar 

  • Chun J (1995) Computer-assisted classification and identification of Actinomycetes. Dissertation, Unversity of Newcastle

  • Dearness J, House HD (1940) New or noteworthy species of fungi. V. Circ. New York State Museum 24:25-60

  • Forsberg LI (1983) New Disease: Alternaria panax on Umbrella tree. Australas Plant Pathol 12:7

    Article  Google Scholar 

  • Gannibal PB, Klemsdal SS, Levitin MM (2007) AFLP analysis of Russian Alternaria tenussima populations from wheat kernels and other hosts. Eur J Plant Pathol 119:175–182

    Article  CAS  Google Scholar 

  • Garibaldi A, Gilardi G, Gullino ML (2004) First Report of Alternaria leaf blight of Aralia japonica caused by Alternaria panax in Europe. Plant Dis 88:82

    Article  Google Scholar 

  • Gherbawy YAMH (2005) Genetic variation among isolates of Altenraria spp. from select Egyptian crops. Arch Phytopathol Plant Protect 38:77–89

    Article  CAS  Google Scholar 

  • Glass NL, Donaldson GC (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 61:1323–1330

    PubMed  CAS  Google Scholar 

  • Greene HC (1953) Notes on Wisconsin parasitic fungi. XVIII. Wis Acad Sci Art Lett 42:69–81

    Google Scholar 

  • Hong SG, Cramer RA, Lawrence CB, Pryor BM (2005) Alt a 1 allergen homologs from Alternaria and related taxa: analysis of phylogenetic content and secondary structure. Fungal Genet and Biol 42:119–129

    Article  CAS  Google Scholar 

  • Hong SG, Maccaroni M, Figuli PJ, Pryor BM, Belisario A (2006) Polyphasic classification of Alternaria isolated from hazelnut and walnut fruit in Europe. Mycol Res 110:1290–1300

    Article  PubMed  CAS  Google Scholar 

  • Kang JC, Crous PW, Mchau GRA, Serdani M, Song SM (2002) Phylogenetic analysis of Alternaria spp. associated with apple core rot and citrus black rot in South Africa. Mycol Res 106:1151–1162

    Article  CAS  Google Scholar 

  • Kim YC, Lee JH, Bae YS, Sohn BK, Park SK (2010) Development of effective environmentally-friendly approaches to control Alternaria blight and anthracnose diseases of Korean ginseng. Eur J Plant Pathol 127:443–450

    Article  Google Scholar 

  • Kumar V, Haldar S, Pandey KK, Singh RP, Singh AK, Singh PC (2008) Cultural, morphological, pathogenic and molecular variability amongst tomato isolates of Alternaria solani in India. World J Microbiol Biotechnol 24:1003–1009

    Article  CAS  Google Scholar 

  • Kusaba M, Tsuge T (1994) Nuclear ribosomal RNA variation and pathogenic specialization in Alternaria fungi known to produce host-specific toxins. Appl Environ Microbiol 60:3055–3062

    PubMed  CAS  Google Scholar 

  • Kusaba M, Tsuge T (1995) Phylogeny of Alternaria fungi known to produce host-specific toxins on the basis of variation in internal transcribed spacers of ribosomal DNA. Curr Genet 28:491–498

    Article  PubMed  CAS  Google Scholar 

  • Leahy RM (1986) Alternaria leaf blight of Brassaia and related hosts. Plant Pathology Circular No. 283, Florida Department of Agriculture and Consumer Services, Division of Plant Industry

  • Levy E, Elkind G, Ben-Ze’ev IS (2006) First report in israel of Aralia leaf spot caused by Alternaria panax. Phytoparasitica 34:269–271

    Article  Google Scholar 

  • Liu YJ, Whelen S, Hall BD (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA Polymerse II Subunit. Mol Biol Evol 16:1799–1808

    Article  PubMed  CAS  Google Scholar 

  • Lourenço V Jr, Moya A, González-Candelas F, Carbone I, Maffia LA, Mizubuti ESG (2009) Molecular diversity and evolutionary processes of Alternaria solani in Brazil inferred using genealogical and coalescent approaches. Phytopathology 99:765–774

    Article  PubMed  Google Scholar 

  • Lourenço V Jr, Rodrigues TTMS, Campos AMD, Bragança CAD, Scheuermann KK, Reis A, Brommonschenkel SH, Maffia LA, Mizubuti ESG (2011) Genetic structure of the population of Alternaria solani in Brazil. Phytopathology 159:233–240

    Google Scholar 

  • Martinez SP, Snowdon R, Pons-Kuhnemann J (2004) Variability of Cuban and international populations of Alternaria solani from different hosts and localities: AFLP genetic analysis. Eur J Plant Pathol 110:399–409

    Article  Google Scholar 

  • McKay GJ, Brown AE, Bjourson AJ, Mercer P (1999) Molecular characterization of Alternaria linicola and its detection in linseed. Eur J Plant Pathol 105:157–166

    Google Scholar 

  • Miller HN (1957). An Alternaria leaf spot of Schefflera actinophylla. Phytopathology 47:529

    Google Scholar 

  • Miller MA, Pfeiffer W, Schwartz T (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceedings of the Gateway Computing Environments Workshop (GCE), New Orleans, pp 1–8

  • Park MS, Seo GS, Bae KS, Yu SH (2005) Characterization of Trichoderma spp. associated with green mold oyster mushroom by PCR-RFLP and sequence analysis of ITS regions of rDNA. Plant Pathol J 21:229–236

    Article  Google Scholar 

  • Park MS, Romanoski CE, Pryor BM (2008) A re-examination of the phylogenetic relationship between the causal agents of carrot black rot, Alternaria radicina and A. carotiincultae. Mycologia 100:511–527

    Article  PubMed  Google Scholar 

  • Parke JL, Shotwell KM (1989) Diseases of cultivated ginseng. Univ Wisconsin Madison Coll Agric Life Sci Res Div Res Bull A3465:16

    Google Scholar 

  • Peever TL, Ibanez A, Akimitsu K, Timmer LW (2002) Worldwide phylogeography of the citrus brown spot pathogen, Alternaria alternata. Phytopathology 92:794–802

    Article  PubMed  CAS  Google Scholar 

  • Peever TL, Su J, Carpenter-Boggs L, Timmer LW (2004) Molecular systematics of citrus-associated Alternaria species. Mycologia 96:119–134

    Article  PubMed  CAS  Google Scholar 

  • Peterson SW (2008) Phylogenetic analysis of Aspergillus species using DNA sequences from four loci. Mycologia 100:205–226

    Article  PubMed  CAS  Google Scholar 

  • Proctor JTA, Baily WG (1987) Ginseng: industry, botany and culture. Hortic Rev 9:187–236

    Google Scholar 

  • Pryor BM, Bigelow DM (2003) Molecular characterization of Embllisia and Nimbya species and their relationship to Altenraria, Ulocladium and Stemphylium. Mycologia 95:1141–1154

    Article  PubMed  CAS  Google Scholar 

  • Pryor BM, Gilbertson RL (2002) Relationships and taxonomic status of Alternaria radicina, A. carotiincultae, and A. petroselini based upon morphological, biochemical, and molecular characteristics. Mycologia 94:49–61

    Article  PubMed  CAS  Google Scholar 

  • Pryor BM, Michailides TJ (2002) Morphological, pathogenic, and molecular characterization of Alternaria isolates associated with Alternaria late blight of pistachio. Phytopathology 92:407–416

    Article  Google Scholar 

  • Putnam ML, du Toit LJ (2003) First report of alternaria blight caused by Alternaria panax on ginseng (Panax quinquefolius) in Oregon and Washington, USA. Plant Pathol 52:406

    Article  Google Scholar 

  • Quayyum HA, Gijzen M, Traquair JA (2003) Purification of a necrosis-inducing, hostspecific protein toxin from spore germination fluid of Alternaria panax. Phytopathology 93:323–328

    Article  PubMed  CAS  Google Scholar 

  • Quayyum HA, Dobinson KF, Traquair JA (2005) Conidial morphology, virulence, molecular characterisation, and host-parasite interactions of selected Alternaria panax isolates on American ginseng. Can J Bot 83:1133–1143

    Article  CAS  Google Scholar 

  • Roberts RG, Reymond ST, Andersen B (2000) RAPD fragment pattern analysis and morphological segregation of small-spored Alternaria species and species groups. Mycol Res 104:151–160

    Article  CAS  Google Scholar 

  • Simmons EG (1982) Alternaria: Themes and Variations (7–10). Mycotaxon 14:17–43

    Google Scholar 

  • Simmons EG (1992) Alternaria taxonomy: Current status, viewpoint, challenge. In: Chelkowski J, Visconti A (eds) Alternaria biology, plant diseases and metabolites. Elsevier, Amsterdam, pp 1–36

    Google Scholar 

  • Simmons EG (2007) ALTERNARIA: an identification manual. CBS Fungal Biodiversity Centre Utrecht, the Netherlands, pp 38–41

    Google Scholar 

  • Stamatakis A (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22:2688–2690

    Article  PubMed  CAS  Google Scholar 

  • Stamatakis A, Hoover P, Rougemont J (2008) A rapid bootstrap algorithm for the RAxML web servers. Syst Biol 57:758–771

    Article  PubMed  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  PubMed  CAS  Google Scholar 

  • Uchida JY, Aragaki M, Yoshimura MA (1984) Alternaria leaf spots of Brassaia actinophylla, Dizygotheca elegantissima, and Tupidanthus calyptratus. Plant Dis 68:447–449

    Google Scholar 

  • Van der Merwe NA, Gryzenhout M, Steenkamp ET, Wingfield BD, Wingfield MJ (2010) Multigene phylogenetic and population differentiation data confirm the existence of a cryptic species within Chrysoporthe cubensis. Fungal Biol 114:966–979

    Article  PubMed  Google Scholar 

  • Van der Waals JE, Korsten L, Slippers B (2004) Genetic diversity among Alternaria solani isolates from potatoes in South Africa. Plant Dis 88:959–964

    Article  Google Scholar 

  • Vos P, Rogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

    Article  PubMed  CAS  Google Scholar 

  • Whetzel HH, Rosenbaum J (1912) The diseases of ginseng and their control. U. S. Dept Agric, Bur Plant Ind, Bulletin No. 250, pp 44

  • White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. New York, pp 253–288

  • Woodhall J, Sansford C (2006) CSL Pest risk analysis for Alternaria panax. Summary pest risk analysis for PHSI interceptions. CSL Sand Hutton, UK

    Google Scholar 

  • Yu SH (2001) Korean Species of Alternaria and Stemphylium. National Institute of Agricultural Science and Technology, Suwon, pp 100–106

    Google Scholar 

  • Yu SH, Syoyo N, Takayuki H (1984) Morphology and Pathogenicity of Alternaria panax isolated from Panax schiseng in Japan and Korea. Ann Phytopath Soc Japan 50:313–321

    Article  Google Scholar 

  • Yu SH, Yun HK, Kim WG, Lee HY, Lee EJ (1992) Alternaria leaf spot of Arlia cordata caused by Alternaria panax. Korean J Plant Pathol 8:81–84

    Google Scholar 

  • Yu SH, Cho HS, Kim BR, Park MS (2003) Morphological and molecular characterization of Alternaria isolates from Solanaceous crops. Korean J Mycol 31:103–113

    Article  Google Scholar 

  • Zaccardelli M, Balmas V, Altomare C, Corazza L, Scotti C (2006) Characterization of Italian isolates of Fusarium semitectum from Alfalfa (Medicago sativa L.) by AFLP analysis, morphology, pathogenicity and toxin production. J Phytophathology 154:454–460

    Article  CAS  Google Scholar 

  • Zhang TY (2003) Flora Fungorum Sinicorum, Alternaria, vol. 16, 1st edn. Beijing, China, pp 55–61

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Acknowledgments

This study was supported by a grant from Regional Subgenebank Support Program of the Rural Development Administration (RDA), Republic of Korea.

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Authors and Affiliations

  1. Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon, 305-764, Korea

    Jian Xin Deng, Narayan Chandra Paul & Seung Hun Yu

  2. Bio-control research team, Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology, Daejeon, 305-600, Korea

    Myung Soo Park

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  1. Jian Xin Deng
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Correspondence to Seung Hun Yu.

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Deng, J.X., Paul, N.C., Park, M.S. et al. Molecular characterization, morphology, and pathogenicity of Alternaria panax from araliaceous plants in Korea. Mycol Progress 12, 383–396 (2013). https://doi.org/10.1007/s11557-012-0844-8

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