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Mass production of medium-chain-length poly(3-hydroxyalkanoates) from hydrolyzed corn oil by fed-batch culture of Pseudomonas putida

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Abstract

A novel biopolyester, Medium-Chain-Length Polyhydroxyalkanoates (MCL-PHAs), was made from the corn oil hydrolysate, an inexpensive and renewable carbon source, by a fed-batch culture of Pseudomonas putida with a phosphate limitation. The cell growth, MCL-PHAs accumulation and feeding strategies of corn oil hydrolysate in the cultures of P. putida were investigated in 5 l and 30 l fermentors respectively. In the optimal fermentation, the final cell and MCL-PHAs concentrations reached 103 and 28 g/l, which represents a MCL-PHAs productivity of 0.61 g/l h. It was confirmed by its NMR spectrum that this MCL-PHAs from corn oil hydrolysate contained 4 saturated and 3 unsaturated monomers with a chain length of 6–14 carbon atoms.

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Acknowledgments

This work was funded by the Ningbo Natural Science Foundation (2006A610043), and the Science and Technology Department of Zhejiang province (2007R10020).

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Correspondence to Longan Shang.

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Shang, L., Jiang, M., Yun, Z. et al. Mass production of medium-chain-length poly(3-hydroxyalkanoates) from hydrolyzed corn oil by fed-batch culture of Pseudomonas putida . World J Microbiol Biotechnol 24, 2783–2787 (2008). https://doi.org/10.1007/s11274-008-9808-1

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  • DOI: https://doi.org/10.1007/s11274-008-9808-1

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