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Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV) with a broad range of 3HV content at high yields by Burkholderia sacchari IPT 189

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Abstract

The biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from sucrose and propionic acid by Burkholderia sacchari IPT 189 was studied using a two-stage bioreactor process. In the first stage, this bacterium was cultivated in a balanced culture medium until sucrose exhaustion. In the second stage, a solution containing sucrose and propionic acid as carbon source was fed to the bioreactor at various sucrose/propionic acid (s/p) ratios at a constant specific flow rate. Copolymers with 3HV content ranging from 40 down to 6.5 (mol%) were obtained with 3HV yield from propionic acid (Y 3HV/prop) increasing from 1.10 to 1.34 g g−1. Copolymer productivity of 1 g l−1 h−1 was obtained with polymer biomass content rising up to 60% by increasing a specific flow rate at a constant s/p ratio. Increasing values of 3HV content were obtained by varying the s/p ratios. A simulation of production costs considering Y 3HV/prop obtained in the present work indicated that a reduction of up to 73% can be reached, approximating US$ 1.00 per kg which is closer to the value to produce P3HB from sucrose (US$ 0.75 per kg).

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Acknowledgments

This work was supported by CNPq and FAPESP, Brazil. The authors thank Aelson L. Santos, Régis N. Carvalho and Valter de Oliveira for technical assistance.

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Authors and Affiliations

  1. Instituto de Pesquisas Tecnológicas do Estado de São Paulo, Laboratório de Biotecnologia Industrial, Av. Prof. Almeida Prado 532, Sao Paulo, 05508-901, SP, Brazil

    Rafael C. S. Rocha, Marilda K. Taciro & José G. C. Pradella

  2. Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes 1374–Biomédicas II, Sao Paulo, 05508-000, SP, Brazil

    Luiziana F. da Silva

Authors
  1. Rafael C. S. Rocha
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  2. Luiziana F. da Silva
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  3. Marilda K. Taciro
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  4. José G. C. Pradella
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Correspondence to Rafael C. S. Rocha.

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Rocha, R.C.S., da Silva, L.F., Taciro, M.K. et al. Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV) with a broad range of 3HV content at high yields by Burkholderia sacchari IPT 189. World J Microbiol Biotechnol 24, 427–431 (2008). https://doi.org/10.1007/s11274-007-9480-x

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  • DOI: https://doi.org/10.1007/s11274-007-9480-x

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