Abstract
All vertebrates possess a daily rhythm, encompassing a comprehensive set of physiological, cognitive, and behavioral patterns that manifest throughout a 24-hour period. The aim of this study was to compare the effect of crude protein (CP) levels in the diet on the daily rhythm of urea in serum (US) and saliva in alpacas and sheep. Ten alpacas and ten sheep, adult, clinically healthy males, were used; they were maintained in natural light conditions and fed ad libitum with two levels of CP (16 and 7%). Blood and saliva samples were taken every 4 h for 48 h. A two-way ANOVA was conducted to analyze the parameters, including adjusted mean rhythm (MESOR), amplitude, and acrophase. It was found that both US and saliva urea exhibited circadian rhythms with a peak during the midday (10:59 − 12:16 h). The MESOR with low CP diets was higher (P < 0.05) in alpacas. The MESOR of urea levels in saliva was greater (P < 0.05) at the highest level of CP in the diet, with no differences between alpacas and sheep (P > 0.05). The amplitude was greater (P < 0.05) in alpacas and at the high level of CP compared to the low level of CP in the diet. Our findings reveal that both serum and saliva urea levels in alpacas and sheep follow a daily rhythm and the MESOR of US was higher in alpacas when they consume food with low CP content, and this difference disappears when CP levels are increased in the diet.
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References
Assenza A, Fazio F, Marcenò G, Piccione G, Caola G (2009) Daily rhythms of serum and salivary parameters in goats. Aust Vet J 87(10):397–401. https://doi.org/10.1111/j.1751-0813.2009.00480.x
Cirio A, Méot F, Delignette-Muller ML, Boivin R (2000) Determination of parotid urea secretion in sheep by means of ultrasonic flow probes and a multifactorial regression analysis. J Anim Sci 78(2):471–476. https://doi.org/10.2527/2000.782471x
Cornelissen G (2014) Cosinor-based Rhythmometry. Theor Biol Med Model 11(1):1–24. https://doi.org/10.1186/1742-4682-11-16
Fawcett JK, Scott JE (1960) A rapid and precise method for the determination of urea. J Clin Pathol 13:156–159. https://doi.org/10.1136/jcp.13.2.156
Folman Y, Neumark H, Kaim M, Kaufmann W (1981) Performance, rumen and blood metabolites in high-yielding cows fed varying protein percents and protected soybean. J Dairy Sci 64(5):759–768. https://doi.org/10.3168/jds.S0022-0302(81)82645-8
Furukawa T, Manabe S, Watanabe T, Sehata S, Sharyo S, Okada T, Mori Y (1999) Daily fluctuation of hepatic p450 monooxygenase activities in male rats is controlled by the suprachiasmatic nucleus but remains unaffected by adrenal hormones. Arch Toxicol 73(7):367–372. https://doi.org/10.1007/s002040050675
Gustafsson AH, Palmquist DL (1993) Diurnal variation of rumen ammonia, serum urea, and milk urea in dairy cows at high and low yields. J Dairy Sci 76(2):475–484. https://doi.org/10.3168/jds.s0022-0302(93)77368-3
Hinderer S, Engelhardt W (1975) Urea metabolism in the Llama. Comp Biochem Physiol Part Mol Integr Physiol 52(4):619–622. https://doi.org/10.1016/S0300-9629(75)80012-0
Lac G (2001) Saliva assays in clinical and research biology. Pathol Biol 49(8):660–667. https://doi.org/10.1016/s0369-8114(01)00228-0
Lefcourt AM, Huntington JB, Akers RM, Wood DL, Bitman J (1999) Circadian and ultradian rhythms of body temperature and peripheral concentrations of insulin and nitrogen in lactating dairy cows. Domest Anim Endocrinol 16(1):41–55. https://doi.org/10.1016/S0739-7240(98)00047-2
Lemosquet S, Dardillat C, Jailler M, Dulphy JP (1996) Voluntary intake and gastric digestion of two hays by llamas and sheep: influence of concentrate supplementation. J Agric Sci 127(4):539–548. https://doi.org/10.1017/s0021859600078771
Michaowski T (1975) Effect of different diets on the diurnal concentrations of ciliate protozoa in the rumen of water buffalo. J Agric Sci 85(1):145–150. https://doi.org/10.1017/S002185960005351X
Olmos-Colmenero JJ, Broderick GA (2006) Effect of dietary crude protein concentration on milk production and nitrogen utilization in lactating dairy cows. j Dairy Sci 89(5):1704–1712. https://doi.org/10.3168/jds.s0022-0302(06)72238-x
Piccione G, Caola G (2002) Biological rhythm in livestock. J Vet Sci (Suwon-Si Korea) 3(3):145–157
Piccione G, Caola G, Refinetti R (2003) Circadian rhythms of body temperature and liver function in fed and food-deprived goats. Comp Biochem Physiol Mol Integr Physiol 134(3):563–572. https://doi.org/10.1016/S1095-6433(02)00362-8
Piccione G, Caola G, Refinetti R (2005) Temporal relationships of 21 physiological variables in horse and sheep. Comp Biochem Physiol Mol Integr Physiol 142(4):389–396. https://doi.org/10.1016/j.cbpa.2005.07.019
Piccione G, Foà A, Bertolucci C, Caola G (2006) Daily rhythm of salivary and serum urea concentration in sheep. J Circadian Rhythms 4:2–5. https://doi.org/10.1186/1740-3391-4-16
Piccione G, Grasso F, Fazio F, Assenza A, Caola G (2007) Influence of different schedules of feeding on daily rhythms of blood urea and ammonia concentration in cows. Biol Rhythm Res 38(2):133–139. https://doi.org/10.1080/09291010600913964
Reynolds CK, Kristensen NB (2008) Nitrogen recycling through the gut and the nitrogen economy of ruminants: an asynchronous symbiosis. J Anim Sci 86(14 Suppl):E293–305. https://doi.org/10.2527/jas.2007-0475
San Martín F, Bryant FC (1989) Nutrition of domesticated South American Llamas and Alpacas. Small Rumin Res 2:191–216. https://doi.org/10.1016/0921-4488(89)90001-1
Tan Z, Murphy MR (2004) Ammonia production, ammonia absorption, and urea recycling in ruminants. A review. J Anim Feed Sci 13(3):389–404. https://doi.org/10.22358/jafs/67425/2004
Wu G (2017) Principles of animal nutrition, 1st edn. CRC Press, Boca Raton, p 772. https://doi.org/10.1201/9781315120065
Zarrin M, Riveros JL, Ahmadpour A, de Almeida AM, Konuspayeva G, Vargas-Bello-Pérez E, Faye B, Hernández-Castellano LE (2020) Camelids: new players in the international animal production context. Trop Anim Health Prod 52(3):903–913. https://doi.org/10.1007/s11250-019-02197-2
Zhang XM, Chen WX, Yan QX, Wang C, Lin B, Yi SY, Wang R, Ma ZY, Li QS, Jonker A, Sun XZ, Wittayakun S, Tan ZL, Wang M (2023) Low-protein diet promotes nitrogen retention efficiency via enhanced renal urea reabsorption and microbial hydrogen incorporation in the rumen of goats. Anim Feed Sci Technol 305(April):115762. https://doi.org/10.1016/j.anifeedsci.2023.115762
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JO: Sample collection, writing – original draft, writing - Reviewing and Editing. LGP: Sample collection, writing – original draft, writing - Reviewing and Editing. FSM: Sample collection and writing – original draft. CG: Data curation and data analysis, Writing - Reviewing and Editing. All authors read and approved the final manuscript..
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Olazabal-Loaiza, J., Gomez-Puerta, L.A., Martín, F.S. et al. Circadian rhythm of salivary and serum urea concentration in alpacas and sheep receiving diets with different levels of protein. Vet Res Commun 48, 2761–2766 (2024). https://doi.org/10.1007/s11259-024-10399-9
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DOI: https://doi.org/10.1007/s11259-024-10399-9