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Evaluation of the protective effect of bovine lactoferrin against lipopolysaccharides in a bovine mammary epithelial cell line

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Abstract

Lactoferrin (Lf) is a non-haem iron-binding glycoprotein with a molecular weight of about 80 kDa, synthesized by glandular epithelial cells and stored in the secondary granules of neutrophils. The physiological significance of Lf is related to non-specific immune defence against pathogens, immunomodulatory activity, iron homeostasis, antioxidant properties and regulation of cell growth. Lf is a bioactive component of the mammary secretions and its modulatory and defensive functions do affect the newborn and the mammary gland as well. In this work a bovine mammary epithelial cell line (BME-UV1) was used as an in vitro model of the bovine mammary epithelium to examine the protective role of exogenous bovine Lf (bLf) against the cytotoxic damage induced by bacterial lipopolysaccharides (LPS) and the endogenous bLf mRNA expression after LPS exposure. In the in vitro model used, exogenous bLf exerts a protective effect against endotoxin cytotoxicity, which could be mediated by the LPS-neutralizing capability of bLf. In addition, in BME-UV1 cells the response to LPS exposure does not involve bLf mRNA expression, suggesting that this cell line lack of functional LPS-responsive elements.

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Acknowledgement

This work was supported by Italian University and Research Grant PRIN 2007.

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Authors and Affiliations

  1. Dipartimento di Scienze e Tecnologie Veterinarie per la Sicurezza Alimentare, Università degli Studi di Milano, via Celoria 10, 20133, Milano, Italy

    Chiara Pecorini, Raffaella Rebucci, Francesca Saccone & Antonella Baldi

  2. Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, Università degli Studi di Milano, via Celoria 10, 20133, Milano, Italy

    Davide Sassera & Claudio Bandi

  3. Dipartimento di Scienze e Tecnologie Veterinarie per la Sicurezza Alimentare, Università degli Studi di Milano, via Trentacoste 2, 20134, Milano, Italy

    Chiara Pecorini

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  1. Chiara Pecorini
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  2. Davide Sassera
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Correspondence to Chiara Pecorini.

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Pecorini, C., Sassera, D., Rebucci, R. et al. Evaluation of the protective effect of bovine lactoferrin against lipopolysaccharides in a bovine mammary epithelial cell line. Vet Res Commun 34, 267–276 (2010). https://doi.org/10.1007/s11259-010-9351-z

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