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Molecular cloning and expression analysis of a F-type lectin gene from Japanese sea perch (Lateolabrax japonicus)

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Abstract

The techniques of homology cloning and anchored PCR were used to clone the fucose-binding lectin (F-type lectin) gene from Japanese sea perch (Lateolabrax Japonicus). The full-length cDNA of sea perch F-lectin (JspFL) contained a 5′ untranslated region (UTR) of 39 bp, an ORF of 933 bp encoding a polypeptide of 310 amino acids with an estimated molecular mass of 10.82 kDa and a 3′ UTR of 332 bp. The searches for nucleotides and protein sequence similarities with BLAST analysis indicated that the deduced amino acid sequence of JspFL was homological to the Fucose-binding lectin in other fish species. In the JspFL deduced amino acid sequence, two tandem domains that exhibit the eel carbohydrate-recognition sequence motif were found. The temporal expressions of gene in the different tissues were measured by real-time PCR. And the mRNA expressions of the gene were constitutively expressed in tissues including spleen, head-kidney, liver, gill, and heart. The JspFL expression in spleen was different during the stimulated time point, 2 h later the expression level became up-regulated, and 6 h later the expression level became down-regulated. The result indicated that JspFL was constitutive and inducible expressed and could play a critical role in the host-pathogen interaction.

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Acknowledgments

This work was supported by a grant from the GuangDong Province of China (2005B20301023).

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Authors and Affiliations

  1. Biotechnology and Aquiculture Laboratory, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 231 Xingangxi Road, Guangzhou, 510300, People’s Republic of China

    Lihua Qiu, Keng Yang, Hanhua Zhang, Jianzhu Li, Falin Zou & Shigui Jiang

  2. Chinese Academy of Fishery Sciences, Beijing, 100039, People’s Republic of China

    Liansheng Lin

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  1. Lihua Qiu
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  2. Liansheng Lin
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  3. Keng Yang
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  4. Hanhua Zhang
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  5. Jianzhu Li
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  7. Shigui Jiang
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Correspondence to Shigui Jiang.

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Lihua Qiu and Liansheng Lin are contributed equally to this work.

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Qiu, L., Lin, L., Yang, K. et al. Molecular cloning and expression analysis of a F-type lectin gene from Japanese sea perch (Lateolabrax japonicus). Mol Biol Rep 38, 3751–3756 (2011). https://doi.org/10.1007/s11033-010-0490-7

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  • DOI: https://doi.org/10.1007/s11033-010-0490-7

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