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Biosynthetic Process and Characterization of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-block-poly(3-hydroxybutyrate) by R. eutropha

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Abstract

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-block-poly(3-hydroxybutyrate) copolymer (P3HBV-b-P3HB) was biosynthesized by addition of pentanoic acid and glucose as carbon sources by Ralstonia eutropha. First, pentanoic acid was metabolized for 72 h to provide P3HBV, and, after changing to a new medium, glucose was used as a second carbon source to give P3HB. In order to regulate the chain length of P3HB, the cultivation time of glucose was varied from 24 to 96 h. The index of randomness of the butyrate and valerate units (D) was estimated based on the resonance line of the carbonyl carbon in the 13C NMR spectrum. The D value was between 3.7 and 9.2 depending on the glucose cultivation time, corresponding to the formation of a block copolymer. The chain lengths of the P3HBV and P3HB blocks were estimated from these values. It was found that as the cultivation time of glucose increased, the chain length of the P3HBV block accumulated at the initial stage decreased, whereas that of the P3HB block increased. This indicates that the P3HBV block was consumed as an energy source for the activity of the microorganism while more of the P3HB block was accumulated, because the end group was active for polymerization.

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References

  1. Kamiya N, Yamamoto Y, Inoue Y, Chujo R, Doi Y (1989) Macromolecules 22:1676

    Article  CAS  Google Scholar 

  2. Holmes PA (1985) Phys Technol 16:32

    Article  CAS  Google Scholar 

  3. Doi Y, Tamaki A, Kunioka M, Soga K (1987) J Chem Soc, Chem Commun 1635

  4. Doi Y, Tamaki A, Kunioka M, Soga K (1998) Appl Microbiol Biotechnol 28:330

    Article  Google Scholar 

  5. Mitomo H, Morishiota N, Doi Y (1993) Macromolecules 26:5809

    Article  CAS  Google Scholar 

  6. Yoshie N, Menju H, Sato H, Inoue Y (1995) Macromolecules 28:6516

    Article  CAS  Google Scholar 

  7. Yoshie N, Fujiwara M, Kasuya K, Abe H, Doi Y, Inoue Y (1993) Macromolecules 26:5809

    Article  Google Scholar 

  8. Yoshie N, Fujiwara M, Kasuya K, Abe H, Doi Y, Inoue Y (1999) Macromol Chem Phys 200:977

    Article  CAS  Google Scholar 

  9. De Smet MJ, Eggink G, Withol B, Kingma J, Wynberg H (1983) J Bacteriol 154:870

    Google Scholar 

  10. Lageveen RG, Huisman GW, Preusting H, Ketelaar P, Eggink G, Witholt B (1988) Appl Environ Microbiol 54:2924

    CAS  Google Scholar 

  11. Brandl H, Gross RA, Renz RW, Fuller RC (1988) Appl Environ Microbiol 54:1977

    CAS  Google Scholar 

  12. Huisman GW, De Leeuw O, Eggink G, Witholt B (1989) Appl Environ Microbiol 55:1949

    CAS  Google Scholar 

  13. Haywood GW, Andersen AJ, Dawes EA (1989) Biotechnol Lett 11:471

    Article  CAS  Google Scholar 

  14. Miura T, Ishii D, Nakaoki T (2013) J Polym Environ 21:760

    Article  CAS  Google Scholar 

  15. Hirt TD, Neuenschwander P, Suter UW (1996) Macromol Chem Phys 197:1609

    Article  CAS  Google Scholar 

  16. Hirt TD, Neuenschwander P, Suter UW (1996) Macromol Chem Phys 197:4253

    Article  CAS  Google Scholar 

  17. Reeve MS, McCarthy SP, Gross RA (1993) Macromolecules 26:888

    Article  CAS  Google Scholar 

  18. Shuai X, Jedlinski Z, Luo Q, Farhod N (2000) Chin J Polym Sci 18:19 (Eng. Ed.)

    CAS  Google Scholar 

  19. Kumagai Y, Doi Y (1993) J Environ Polym Degrad 1:81

    Article  CAS  Google Scholar 

  20. Li X, Loh XJ, Wang K, He CB, Li J (2005) Biomacromolecules 6:2740

    Article  CAS  Google Scholar 

  21. Pederson N, McChallcher CWJ, Snenc F (2006) Biomacromolecules 7:1904

    Article  CAS  Google Scholar 

  22. Mantzaris NV, Kelley AS, Daoutidis P, Srienc F (2002) Chem Eng Sci 57:4643

    Article  CAS  Google Scholar 

  23. Mantzaris NV, Kelley AS, Srienc F, Daoutidis P (2001) AIChE J 47:727

    Article  CAS  Google Scholar 

  24. Li SY, Dong CL, Wang SY, Ye HM, Chen GQ (2011) Appl Microbiol Biotechnol 90:659

    Article  CAS  Google Scholar 

  25. Hu D, Chung A-L, Wu L-P, Zhang X, Wu Q, Chen J-C, Chen G-Q (2011) Biomacromolecules 12:3166

    Article  CAS  Google Scholar 

  26. Tripathi L, Wu LP, Chen GQ (2012) Microb Cell Fact 11:44

    Article  CAS  Google Scholar 

  27. Tappel RC, Kucharski JM, Mastroianni JM, Stipanovic AJ, Nomura CT (2012) C. Biomacromolecules 13:2964

    Article  CAS  Google Scholar 

  28. Wang Q, Yang P, Xian M, Liu H, Cao Y, Yang Y, Zhao G, Macro ACS (2013) Letters 2:996

    CAS  Google Scholar 

Download references

Acknowledgments

This work was partially supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan (No. 24550179). In addition, financial support from a research fund at Ryukoku University is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Materials Chemistry, Ryukoku University, Seta, Otsu, 520-2194, Japan

    Takahiko Nakaoki, Risa Yamagishi & Daisuke Ishii

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  1. Takahiko Nakaoki
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  2. Risa Yamagishi
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  3. Daisuke Ishii
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Correspondence to Takahiko Nakaoki.

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Nakaoki, T., Yamagishi, R. & Ishii, D. Biosynthetic Process and Characterization of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-block-poly(3-hydroxybutyrate) by R. eutropha . J Polym Environ 23, 487–492 (2015). https://doi.org/10.1007/s10924-015-0735-6

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  • DOI: https://doi.org/10.1007/s10924-015-0735-6

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