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Meristiella echinocarpa lectin (MEL): a new member of the OAAH-lectin family

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Abstract

A new lectin from the marine red alga Meristiella echinocarpa (MEL) was isolated and biochemically characterized. MEL is a monomeric protein of 28 kDa with specificity for yeast mannan. Hemagglutination activity of MEL was stable between pH 5 and 10, temperatures up to 50 °C, and neither EDTA nor divalent ions affected it. The complete amino acid sequence of MEL was determined through a combination of tandem mass spectrometry and DNA cloning. As a new member of the OAAH-lectin family, the primary structure of MEL consists of 267 amino acid residues distributed in four tandem repeat domains, sharing at least 48% of identity. Theoretical secondary structure of MEL was composed of 3% α-helix, 40% β-sheet, 19% β-turn, and 38% coil. Melting temperatures of the lectin in the absence and presence of mannan were 54 and 61 °C, respectively. Furthermore, MEL was able to recognize and agglutinate pathogenic bacterial strains, such as multidrug-resistant Salmonella and Vibrio alginolyticus.

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Acknowledgments

This work was supported by the Brazilian agencies CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), FUNCAP (Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico), and FINEP (Financiadora de Estudos e Projetos). The authors thank CETENE by the availability of the mass spectrometer, and, especially, the authors are grateful to Dr. Julia Campos for MALDI-ToF experiments. AHS, CSN, and OVS are senior investigators of CNPq.

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Authors and Affiliations

  1. BioMar-Lab, Departamento de Engenharia de Pesca, Universidade Federal do Ceará, Campus do Pici s/n, Av. Mister Hull, bloco 871, Fortaleza, Ceará, 60440-970, Brazil

    Renata Pinheiro Chaves, João Pedro Freire Alves da Silva, Rômulo Farias Carneiro, Wladimir Ronald Lobo Farias, Alexandre Holanda Sampaio & Celso Shiniti Nagano

  2. Campus Monte Alegre, Universidade Federal do Oeste do Pará, Av. Major Francisco Mariano s/n - Bairro Cidade Alta, Monte Alegre, PA, 68220-000, Brazil

    Suzete Roberta da Silva

  3. Faculdade de Filosofia Dom Aureliano Matos, Universidade Estadual do Ceará, Av. Dom Aureliano Matos, 2060, Limoeiro do Norte, CE, 62930-000, Brazil

    Bruno Lopes de Sousa

  4. Instituto de Ciências do Mar – Labomar, Universidade Federal do Ceará, Av. da Abolição, 3207, Fortaleza, CE, 60165-081, Brazil

    Jade Oliveira Abreu, Fátima Cristiane Teles de Carvalho & Oscarina Viana de Sousa

  5. Departamento de Bioquímica, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego, 1235, Cidade Universitária, Recife, PE, 50670-901, Brazil

    Cintia Renata Costa Rocha

  6. Departamento de Bioquímica, Universidade Federal do Ceará, Campus do Pici s/n bloco 907, Fortaleza, CE, 60440-970, Brazil

    André Luiz Coelho Silva

Authors
  1. Renata Pinheiro Chaves
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  2. Suzete Roberta da Silva
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  3. João Pedro Freire Alves da Silva
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  4. Rômulo Farias Carneiro
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  7. Fátima Cristiane Teles de Carvalho
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  8. Cintia Renata Costa Rocha
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  9. Wladimir Ronald Lobo Farias
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  10. Oscarina Viana de Sousa
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  11. André Luiz Coelho Silva
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  12. Alexandre Holanda Sampaio
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  13. Celso Shiniti Nagano
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Corresponding author

Correspondence to Celso Shiniti Nagano.

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This work is dedicated in memory of Wladimir Ronald Lobo Farias.

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Chaves, R.P., da Silva, S.R., da Silva, J.P.F.A. et al. Meristiella echinocarpa lectin (MEL): a new member of the OAAH-lectin family. J Appl Phycol 30, 2629–2638 (2018). https://doi.org/10.1007/s10811-018-1473-7

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