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Isorhapontigenin Suppresses Interleukin-1β-Induced Inflammation and Cartilage Matrix Damage in Rat Chondrocytes

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Abstract

Osteoarthritis (OA) is a common cause of joint pain and physical disability in the elderly. It is highly associated with local inflammatory reactions and cartilage degradation. Isorhapontigenin (ISO), a natural compound existing in various plants, has shown prominent anti-inflammatory and anti-oxidative properties in several inflammatory diseases. However, the effects of ISO on OA remain to be elucidated. Here, we investigated the effects of ISO on interleukin-1β (IL-1β)-treated rat chondrocytes and cartilage explants. Our results revealed that ISO could suppress the IL-1β-induced elevated levels of nitric oxide (NO), inducible nitric oxide synthase (iNOS), prostaglandin E2 (PGE2), and cyclooxygenase-2 (COX2). Besides, ISO could also inhibit the IL-1β-induced up-regulation of cartilage matrix catabolic enzymes such as matrix metalloproteinases (MMPs) and aggrecanase-2 (ADAMTS5). Moreover, the IL-1β-induced downregulation of collagen II and aggrecan could be reversed by ISO. Furthermore, ISO prevented rat cartilage explant damage induced by IL-1β. Mechanistically, ISO worked partly by suppressing mitogen-activated protein kinase (MAPK)-associated ERK and p38 pathways. Taken together, our study indicated the anti-inflammatory potential of ISO on IL-1β-treated rat chondrocytes, providing a new idea for OA treatment.

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Acknowledgments

This study was supported by the grant from the National Natural Science Foundation of China (no. 51537004) to H.W.

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Author notes

  1. Yongzhuang Ma and Chang Tu contributed equally to this work.

Authors and Affiliations

  1. Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, People’s Republic of China

    Yongzhuang Ma, Chang Tu & Hua Wu

  2. Department of Pathology and Pathophysiology, School of Medicine, Jianghan University, Wuhan, Hubei, People’s Republic of China

    Wei Liu & Yifan Xiao

Authors
  1. Yongzhuang Ma
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  2. Chang Tu
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  3. Wei Liu
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  4. Yifan Xiao
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  5. Hua Wu
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Corresponding authors

Correspondence to Yifan Xiao or Hua Wu.

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This study was in strict accordance with the Guidelines of Animal Care and Use Committee for Teaching and Research of Huazhong University of Science and Technology, Wuhan, China.

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The authors have no conflict of interest or financial disclosures with this research.

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Ma, Y., Tu, C., Liu, W. et al. Isorhapontigenin Suppresses Interleukin-1β-Induced Inflammation and Cartilage Matrix Damage in Rat Chondrocytes. Inflammation 42, 2278–2285 (2019). https://doi.org/10.1007/s10753-019-01092-0

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  • DOI: https://doi.org/10.1007/s10753-019-01092-0

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