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Generation of stable concentration gradients in 2D and 3D environments using a microfluidic ladder chamber

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Abstract

We have developed a simple microfluidic device for generating stable concentration gradients in 2D and 3D environments. The device, termed the Ladder Chamber, uses a two-compartment diffusion system to generate steady state gradients across flow-free channels that connect the source and sink channels. To demonstrate the utility of the Ladder Chamber for cell migration, neutrophil chemotaxis was successfully observed in soluble chemoattractant (IL-8) gradient. The Ladder Chamber’s simple design and experimental implementation make it an attractive approach for investigating cell migration and other biological experiments in well-defined gradients in 2D surfaces as well as in 3D gels.

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Acknowledgment

We thank Professor Steven S. George and Justin Mih for reagents and valuable discussions. We also thank Dr. Kapil Krishan for assistance with diffusion calculations, and Dr. Mike Papac for equipment. This research was supported by the Department of Defense (Grants no. DAMD17-03-1-0515 and no. DAMD17-03-1-0673).

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Authors and Affiliations

  1. Department of Biomedical Engineering, University of California at Irvine, 3120 Natural Science II, Irvine, CA, 92697, USA

    Wajeeh Saadi, Francis Lin, Behrad Vahidi, Bong Geun Chung & Noo Li Jeon

  2. Department of Chemistry, Kongju National University, 182 Shinkwandong, Kongju, 314-701, South Korea

    Seog Woo Rhee

  3. Department of Physics and Astronomy, University of California at Irvine, Irvine, CA, 92697, USA

    Francis Lin

Authors
  1. Wajeeh Saadi
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  2. Seog Woo Rhee
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  3. Francis Lin
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  4. Behrad Vahidi
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  5. Bong Geun Chung
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  6. Noo Li Jeon
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Corresponding author

Correspondence to Seog Woo Rhee.

Additional information

Equal contribution from Saadi and Rhee.

Supporting Information

Movie of neutrophil migration in a 0–50 ng/mL IL-8 gradient inside microgrooves will be available in supporting information.

10544_2007_9051_MOESM1_ESM.avi

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Saadi, W., Rhee, S.W., Lin, F. et al. Generation of stable concentration gradients in 2D and 3D environments using a microfluidic ladder chamber. Biomed Microdevices 9, 627–635 (2007). https://doi.org/10.1007/s10544-007-9051-9

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  • DOI: https://doi.org/10.1007/s10544-007-9051-9

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