[go: up one dir, main page]
More Web Proxy on the site http://driver.im/ Skip to main content
Springer Nature Link
Log in

Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Cupriavidus necator from waste rapeseed oil using propanol as a precursor of 3-hydroxyvalerate

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Waste rapeseed oil is a useful substrate for polyhydroxyalkanoates (PHA) production employing Cupriavidus necator H16. In fed-batch mode, we obtained biomass and PHA yields of 138 and 105 g l−1, respectively. Yield coefficient and volumetric productivity were 0.83 g PHA per g oil and 1.46 g l−1 h−1, respectively. Propanol at 1% (v/v) enhanced both PHA and biomass formation significantly and, furthermore, resulted in incorporation of 3-hydroxyvalerate units into PHA structure. Thus, propanol can be used as an effective precursor of 3-hydroxyvalarete for production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer. During the fed-batch cultivation, propanol concentration was maintained at 1% which resulted in 8% content of 3-hydroxyvalerate in copolymer.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
£29.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (United Kingdom)

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Brandl H, Gross RA, Lenz RW, Fuller RC (1988) Pseudomonas oleovorans as a source of poly(beta-hydroxyalkanoates) for potential application as a biodegradable polyester. Appl Environ Microb 54:1977–1982

    CAS  Google Scholar 

  • Chan PL, Yu V, Wai L, Yu HF (2006) Production of medium-chain-length polyhydroxyalkanoates by Pseudomonas aeruginosa with fatty acids as alternative carbon sources. Appl Biochem Biotechnol 129:933–941

    Article  PubMed  Google Scholar 

  • Du GC, Chen J, Yu J, Lun S (2001) Feeding strategy of propionic acid for production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with Ralstonia eutropha. Biochem Eng J 8:103–110

    Article  CAS  Google Scholar 

  • Kahar P, Tsuge T, Taguchi K, Doi Y (2004) High yield production of polyhydroxyalkanoates from soybean oil by Ralstonia eutropha and its recombinant strain. Pol Degrad Stabil 83:79–86

    Article  CAS  Google Scholar 

  • Kessler B, Witholt B (1999) Poly(3-hydroxyalkanoates) In: Flickinger MC, Drew SW (eds) Encyclopedia of bioprocess technology—fermentation, biocatalysis and bioseparation. Wiley, New York, pp 2024–2040

  • Lee SY, Choi J (1999) Effect of fermentation performance on the economics of poly-(3-hydroxybutyrate) production by Alcaligenes latus. Pol Degrad Stabil 59:387–393

    Article  Google Scholar 

  • Matthäus B (2007) Use of palm oil for frying in comparison with other high-stability oils. Eur J Lipid Sci Technol 109:400–409

    Article  Google Scholar 

  • Obruca S, Marova I, Svoboda Z, Mikulikova R (2010a) Use of controlled exogenous stress for improvement of poly(3-hydroxybutyrate) production in Cupriavidus necator. Folia Microbiol 55:17–22

    Article  CAS  Google Scholar 

  • Obruca S, Marova I, Stankova M, Mravcova L, Svoboda Z (2010b) Effect of ethanol and hydrogen peroxide on poly(3-hydroxybutyrate) biosynthetic pathway in Cupriavidus necator H16. World J Microb Biot 26:1261–1267

    Article  CAS  Google Scholar 

  • Slater S, Houmiel KL, Tran M, Mitsky TA, Taylor NB, Padgette SR, Gruys KJ (1998) Multiple beta-ketothiolases mediate poly(beta-hydroxyalkanoate) copolymer synthesis in Ralstonia eutropha. J Bacteriol 180:1979–1987

    CAS  PubMed  Google Scholar 

  • Taniguchi I, Kagotani K, Kimura Y (2003) Microbial production of poly(hydroxyalkanoates) from waste edible oils. Green Chem 5:545–548

    Article  CAS  Google Scholar 

  • Vidal-Mas J, Resina-Pelfort O, Haba E, Comas J, Manresa A, Vives-Rego J (2001) Rapid flow cytometry—Nile red assesment of PHA cellular content and heterogeneity in cultures of Pseudomonas aeruginosa 47T2 (NCIB 40044) grown in waste frying oil. Anton Leeuw Int J G 80:57–63

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by projects MSM 0021630501 and CZ.1.05/2.1.00/01.0012 of the Czech Ministry of Education.

Author information

Authors and Affiliations

  1. Faculty of Chemistry, Department of Food Chemistry and Biotechnology, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic

    Stanislav Obruca, Ivana Marova & Ondrej Snajdar

  2. Faculty of Chemistry, Department of Chemistry and Technology of Environmental Protection, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic

    Ludmila Mravcova

  3. Research Institute of Brewing and Malting, Malting Institute Brno, Mostecka 7, 614 00, Brno, Czech Republic

    Zdenek Svoboda

Authors
  1. Stanislav Obruca
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ivana Marova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ondrej Snajdar
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ludmila Mravcova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zdenek Svoboda
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Stanislav Obruca.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Obruca, S., Marova, I., Snajdar, O. et al. Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Cupriavidus necator from waste rapeseed oil using propanol as a precursor of 3-hydroxyvalerate. Biotechnol Lett 32, 1925–1932 (2010). https://doi.org/10.1007/s10529-010-0376-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-010-0376-8

Keywords