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Production of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from Gluconate and Glucose by Recombinant Aeromonas hydrophila and Pseudomonas putida

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Abstract

Aeromonas hydrophila 4AK4 and Pseudomonas putida GPp104 were genetically engineered to synthesize poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) using gluconate and glucose rather than fatty acids. A truncated tesA gene, encoding cytosolic thioesterase I of Escherichia coli which catalyzes the conversion of acyl-ACP into free fatty acids, was introduced into A. hydrophila 4AK4. When grown in gluconate, the recombinant A. hydrophila 4AK4 synthesized 10% (w/w) PHBHHx containing 14% (mol/mol) 3-hydroxyhexanoate. If additional PHBHHx synthesis genes, phaPCJ, were over-expressed with the truncated tesA in A. hydrophila 4AK4, the PHBHHx content increased to 15% (w/w) and contained 19% (mol/mol) 3-hydroxyhexanoate. Recombinant P. putida GPp104 harboring phaC encoding PHBHHx synthase of A. hydrophila, phaB encoding acetoacetyl-CoA reductase of Wautersia eutropha and phaG encoding 3-hydroxyacyl-ACP-CoA transferase of P. putida, synthesized 19% (w/w) PHBHHx containing 5% (mol/mol) 3-hydroxyhexanoate from glucose. The results suggest that the engineered pathways were applicable to synthesize PHBHHx from unrelated carbon sources such as gluconate and glucose.

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Authors and Affiliations

  1. Department of Biological Sciences and Biotechnology, Tsinghua University, 100084, Beijing, China

    Yuan-Zheng Qiu, Jing-Jing Guo & Guo-Qiang Chen

  2. School of Life Science, Shandong Normal University, 250014, Jinan, China

    Jing Han

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  1. Yuan-Zheng Qiu
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  2. Jing Han
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  3. Jing-Jing Guo
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  4. Guo-Qiang Chen
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Correspondence to Guo-Qiang Chen.

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Qiu, YZ., Han, J., Guo, JJ. et al. Production of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from Gluconate and Glucose by Recombinant Aeromonas hydrophila and Pseudomonas putida. Biotechnol Lett 27, 1381–1386 (2005). https://doi.org/10.1007/s10529-005-3685-6

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  • DOI: https://doi.org/10.1007/s10529-005-3685-6

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