[go: up one dir, main page]
More Web Proxy on the site http://driver.im/ Skip to main content
Springer Nature Link
Log in

Helicobacter pylori Virulence Genotypes in Portuguese Children and Adults with Gastroduodenal Pathology

  • Article
  • Published:
European Journal of Clinical Microbiology and Infectious Diseases Aims and scope Submit manuscript

Abstract

The aim of this study was to evaluate the prevalence of virulence genotypes, namely cagA, vacA and babA2, of Helicobacter pylori strains isolated from Portuguese adults and children presenting gastroduodenal pathology. One hundred thirty-six strains were studied, 82 isolated from adult patients (50 with nonulcerative gastritis and 32 with active peptic ulcer) and 58 isolated from children (54 with nonulcerative gastritis and 4 with duodenal ulcer). Genotyping of cagA, vacA and babA2 was assessed by polymerase chain reaction. Overall, Helicobacter pylori strains carrying more virulent genotypes were much more prevalent in adults than in children, particularly the type I (vacAs1- and cagA-positive) and the triple-positive (vacAs1-, cagA- and babA2-positive) strains (P<0.001). A subpopulation of adults and children with nonulcerative gastritis was also studied, and differences in the prevalence of virulent genotypes were observed, either for individual genotypes (P=0.017 for cagA, P=0.010 for vacAs1) or in combinations, i.e. the type I genotype (P=0.005) and the triple-positive strains (P=0.031). There was no difference between the two populations in the distribution of babA2 and m1/m2 genotypes. Considering the cohort effect in the epidemiology of Helicobacter pylori infection, these results suggest that different strains might circulate during different periods of time, or that, after infection in childhood, individual strains will undergo changes during the course of infection.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
£29.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (United Kingdom)

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Blaser MJ (1997) Ecology of Helicobacter pylori in the human stomach. J Clin Invest 100:759–762

    CAS  PubMed  Google Scholar 

  2. Dunn BE, Cohen H, Blaser MJ (1997) Helicobacter pylori. Clin Microbiol Rev 10:720–741

    CAS  PubMed  Google Scholar 

  3. Atherton JC (1997) The clinical relevance of strain types of Helicobacter pylori. Gut 40:701–703

    CAS  PubMed  Google Scholar 

  4. Go MF (1997) What are the host factors that place an individual at risk for Helicobacter pylori-associated disease? Gastroenterology 113 (Suppl 6):15–20

  5. Fox JG, Dangler CA, Taylor NS, King A, Koh TJ, Wang TC (1999) High-salt diet induces gastric epithelial hyperplasia and parietal cell loss, and enhances Helicobacter pylori colonization in C57BL/6 mice. Cancer Res 59:4823–4828

    CAS  PubMed  Google Scholar 

  6. Censini S, Lange C, Xiang Z, Crabtree JE, Ghiara P, Borodovsky M, Rappuoli R, Covacci A (1996) cag, a pathogenicity island of Helicobacter pylori, encodes type-I-specific and disease-associated virulence factors. Proc Natl Acad Sci USA 93:14648–14653

    CAS  PubMed  Google Scholar 

  7. Stein M, Rappuoli R, Covacci A (2000) Tyrosine phosphorylation of the Helicobacter pylori CagA antigen after cag-driven host cell translocation. Proc Natl Acad Sci USA 97:1263–1268

    Article  CAS  PubMed  Google Scholar 

  8. Cover TL (1996) The vacuolating cytotoxin of Helicobacter pylori. Mol Microbiol 20:241–246

    CAS  PubMed  Google Scholar 

  9. Atherton JC, Cao P, Peek RM, Tummuru MKR, Blaser MJ, Cover TL (1995) Mosaicism in the vacuolating cytotoxin alleles of Helicobacter pylori. J Biol Chem 270:17771–17777

    CAS  PubMed  Google Scholar 

  10. van Doorn LJ, Figueiredo C, Sanna R, Pena S, Midolo P, Ng EKW, Atherton JC, Blaser MJ, Quint MGV (1998) Expanding allelic diversity of Helicobacter pylori vacA. J Clin Microbiol 36:2597–2603

    PubMed  Google Scholar 

  11. Blaser MJ, Perez PG, Kleanthous H, Cover TL, Peek RM, Chyou PH, Stemmermann GN, Nomura A (1995) Infection with Helicobacter pylori strains possessing cagA is associated with increased risk of developing adenocarcinoma of the stomach. Cancer Res 55:2111–2115

    CAS  PubMed  Google Scholar 

  12. Parsonnet J, Friedman GD, Orentreich N, Vogelmann H (1997) Risk for gastric cancer in people with CagA positive or CagA negative Helicobacter pylori infection. Gut 40:297–301

    CAS  PubMed  Google Scholar 

  13. Atherton JC, Peek RMJ, Tham KT, Cover TL, Blaser MJ (1997) Clinical and pathological importance of heterogeneity in vacA, the vacuolating cytotoxin gene of Helicobacter pylori. Gastroenterology 112:92–99

    CAS  PubMed  Google Scholar 

  14. Boren T, Falk P, Roth KA, Larson G, Norwark S (1993) Attachment of Helicobacter pylori to human gastric epithelium mediated by blood group antigens. Science 262:1892–1895

    PubMed  Google Scholar 

  15. Ilver D, Arnqvist A, Ogren J, Frick IM, Kersulyte D, Incecik ET, Berg DE, Covacci A, Engstrand L, Boren T (1998) Helicobacter pylori adhesin binding fucosylated histo-blood group antigens revealed by retagging. Science 279:373–377

    CAS  PubMed  Google Scholar 

  16. Gerhard M, Lehn N, Neumayer N, Boren T, Rad R, Schepp W, Miehlke S, Classen M, Prinz C (1999) Clinical relevance of the Helicobacter pylori gene for blood-group antigen-binding adhesin. Proc Natl Acad Sci USA 96:12778–12783

    Article  CAS  PubMed  Google Scholar 

  17. Yamaoka Y, Kodama T, Gutierrez O, Kim JG, Kashima K, Graham DY (1999) Relationship between Helicobacter pylori iceA, cagA and vacA status and clinical outcome: studies in four different countries. J Clin Microbiol 37:2274–2279

    CAS  PubMed  Google Scholar 

  18. van Doorn LJ, Figueiredo C, Megraud F, Pena S, Midolo P, Queiroz DM, Carneiro F, Vanderborght B, Pegado MD, Sanna R, De Boer W, Schneeberger PM, Correa P, Ng EK, Atherton J, Blaser MJ, Quint WG (1999) Geographic distribution of vacA allelic types of Helicobacter pylori. Gastroenterology 116:823–830

    PubMed  Google Scholar 

  19. Achtman M, Azuma T, Berg DE, Ito Y, Morelli G, Pan ZJ, Suerbaum S, Thompson SA, van der Ende A, van Doorn LJ (1999) Recombination and clonal groupings within Helicobacter pylori from different geographical regions. Mol Microbiol 32:459–470

    CAS  PubMed  Google Scholar 

  20. Kersulyte D, Mukhopadhyay AK, Velapatino B, Su W, Pan Z, Garcia C, Hernandez V, Valdez Y, Mistry RS, Gilman RH, Yuan Y, Gao H, Alarcon T, Lopez-Brea M, Balakrish Nair G, Chowdhury A, Datta S, Shirai M, Nakazawa T, Ally R, Segal I, Wong BC, Lam SK, Olfat FO, Boren T, Engstrand L, Torres O, Schneider R, Thomas JE, Czinn S, Berg DE (2000) Differences in genotypes of Helicobacter pylori from different human populations. J Bacteriol 182:3210–3218

    Article  CAS  PubMed  Google Scholar 

  21. Loeb M, Jayaratne P, Jones N, Sihoe A, Sherman P (1998) Lack of correlation between vacuolating cytotoxin activity,cagA gene in Helicobacter pylori and peptic ulcer disease in children. Eur J Clin Microbiol Infect Dis 17:653–656

    Article  CAS  PubMed  Google Scholar 

  22. Mitchell HZ, Hazzel SL, Bohane TD, Hu P, Chen M, Li YY (1999) The prevalence of antibody to CagA in children is not a marker for specific disease. J Pediatr Gastroenterol Nutr 28:71–75

    Article  CAS  PubMed  Google Scholar 

  23. Kato S, Sugiyama T, Kudo M, Ohnuma K, Ozawa K, Iinuma K, Asaka M, Blaser MJ (2000) CagA antibodies in Japanese children with nodular gastritis or peptic ulcer disease. J Clin Microbiol 38:68–70

    CAS  PubMed  Google Scholar 

  24. Gold BD, van Doorn LJ, Guarner J, Owens M, Pierce-Smith D, Song Q, Hutwagner L, Sherman PM, de Mola OL, Czinn SJ (2001) Genotypic, clinical and demographic characteristics of children infected with Helicobacter pylori. J Clin Microbiol 39:1348–1352

    Article  CAS  PubMed  Google Scholar 

  25. Yahav J, Fradkin A, Weisselberg B, Diver-Haver A, Shmuely H, Jonas A (2000) Relevance of CagA positivity to clinical course of Helicobacter pylori infection in children. J Clin Microbiol 38:3534–3537

    CAS  PubMed  Google Scholar 

  26. Queiroz DMM, Mendes EN, Carvalho AST, Rocha GA, Oliveira AMR, Soares TF, Santos A, Cabral MMDA, Nogueira AMMF (2000) Factors associated with Helicobacter pylori infection by a cagA-positive strain in children. J Infect Dis 181:626–630

    Article  CAS  PubMed  Google Scholar 

  27. de Gusmão VR, Nogueira Mendes E, De Magalhães Queiroz DM, Aguiar Rocha G, Camargos Rocha AM, Ramadan Ashour AA, Teles Carvalho AS (2000) vacA genotypes in Helicobacter pylori strains isolated from children with and without duodenal ulcer in Brazil. J Clin Microbiol 38:2853–2857

    PubMed  Google Scholar 

  28. Alarcon T, Domingo D, Martinez MJ, Lopez-Brea M (1999) cagA gene and vacA alleles in Spanish Helicobacter pylori clinical isolates from patients at different ages. FEMS Immunol Med Microbiol 24:215–219

    Article  CAS  PubMed  Google Scholar 

  29. Karhukorpi J, Yan Y, Kolho K-L, Rautelin H, Lathi M, Sirvio A, Riipinen K, Lindahl H, Verkasalo M, Fagerholm R, Kartunen R (2000) cagA, vacA and iceA virulence genes of Helicobacter pylori isolates of children in Finland. Eur J Clin Microbiol Infect Dis 19:790–793

    Article  CAS  PubMed  Google Scholar 

  30. Pitcher DG, Saunders NA, Owen RJ (1989) Rapid extraction of bacterial genomic DNA with guanidinium thiocyanate. Lett Appl Microbiol 8:151–156

    CAS  Google Scholar 

  31. Atherton JC, Cover TL, Twells, Morales MR, Hawkey CJ, Blaser MJ (1999) Simple and accurate PCR-based system for typing vacuolating cytotoxin alleles of Helicobacter pylori. J Clin Microbiol 37:2979–2982

    CAS  PubMed  Google Scholar 

  32. Tummuru MKR, Cover TL, Blaser MJ (1993) Cloning and expression of a high-molecular-mass major antigen of Helicobacter pylori: Evidence of linkage to cytotoxin production. Infect Immun 61:1799–1809

    CAS  PubMed  Google Scholar 

  33. Alarcon T, Domingo D, Martinez M, de la Obra P, Lopez-Brea M (2000) cagA and vacA genes in Helicobacter pylori clinical isolates from a Spanish paediatric population according to age. Gut 47 [Suppl I]:A97

  34. Yamaoka Y, Arnquist A, Borén T, Gutierrez O, Kim JG, Graham DY (1999) Helicobacter pylori blood group antigen-binding adhesin, BabA, and clinical outcome: studies in four different countries. Gut 45 [Suppl III]:A25

  35. Gonzalez-Valencia G, Atherton JC, Munõz O, Dehesa M, de la Garza M, Torres J (2000) Helicobacter pylori vacA and cagA genotypes in Mexican adults and children. J Infect Dis 182:1450–1454

    Article  CAS  PubMed  Google Scholar 

  36. Çelik J, Su B, Tirén U, Finkel Y, Thoresson A-C, Engstrand L, Sandstedt B, Bernander S, Norwark S (1998) Virulence and colonization-associated properties of Helicobacter pylori isolated from children and adolescents. J Infect Dis 177:247–252

    PubMed  Google Scholar 

  37. Blom J, Gernow S, Holck S, Wewer V, Norgaard A, Graff LB, Krasilnikoff, Anderson LP, Larsen SO (2000) Different patterns of Helicobacter pylori adherence to gastric mucosa cells in children and adults. Scand J Gastroenterol 35:1033–1040

    Article  CAS  PubMed  Google Scholar 

  38. Kersulyte D, Chalkauskas H, Berg DE (1999) Emergence of recombinant strains of Helicobacter pylori during human infection. Mol Microbiol 31:31–43

    CAS  PubMed  Google Scholar 

Download references

Acknowledgements

The authors thank Mrs. K. Mayo for revision of the English of the text.

Author information

Authors and Affiliations

  1. Laboratório de Bacteriologia, Instituto Nacional Saúde Dr Ricardo Jorge, Av. Padre Cruz, 1649-016, Lisboa, Portugal

    M. Oleastro & L. Monteiro

  2. Department of Medicine II, Technical University of Munich, Ismaninger Strasse 22, 81675, Munich, Germany

    M. Gerhard

  3. Unidade de Gastrenterologia Pediátrica, Hospital de Santa Maria, Av. Prof. Egas Moniz, 1649-028, Lisboa, Portugal

    A. I. Lopes & P. Ramalho

  4. Unidade de Gastrenterologia-Hepatologia Infantil, Hospital D. Estefânia, R. Jacinta Marto, 1100, Lisboa, Portugal

    J. Cabral

  5. Clínica Universitária de Medicina Interna e Gastrenterologia, Hospital de Pulido Valente, Al. Linhas Torres 117, 1769-001, Lisboa, Portugal

    A. Sousa Guerreiro

Authors
  1. M. Oleastro
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Gerhard
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. I. Lopes
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. P. Ramalho
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. Cabral
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. Sousa Guerreiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. L. Monteiro
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. Oleastro.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Oleastro, M., Gerhard, M., Lopes, A.I. et al. Helicobacter pylori Virulence Genotypes in Portuguese Children and Adults with Gastroduodenal Pathology. Eur J Clin Microbiol Infect Dis 22, 85–91 (2003). https://doi.org/10.1007/s10096-002-0865-3

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10096-002-0865-3

Keywords

Search

Navigation