[go: up one dir, main page]
More Web Proxy on the site http://driver.im/ Skip to main content
Springer Nature Link
Log in

Primary mouse dermal fibroblast cell cultures as an in vitro model system for the differential pathogenicity of cross-species herpesvirus papio 2 infections

  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary.

Infection of mice with herpesvirus papio 2 (HVP2) parallels zoonotic monkey B virus infections. A major benefit of the HVP2/mouse model is the existence of two HVP2 subtypes: HVP2nv rapidly invades and destroys the CNS while HVP2ap produces no clinical signs and mild histopathological lesions. However, in the natural baboon host, no difference in pathogenicity of HVP2 subtypes is evident. Primary dermal fibroblast cells were evaluated as a model system for defining virus-host interactions that influence the outcome of a cross-species infection. No differences in plaque formation or virus replication were observed between HVP2 subtypes in primary baboon dermal fibroblast cultures. In contrast, when primary mouse dermal fibroblasts (PMDF) were infected, HVP2nv replicated to higher titers and was more efficient at shutting down host-cell protein synthesis compared to HVP2ap. HVP2ap-infected PMDF cells produced more IFN-β compared to HVP2nv, and IFN-β pretreatment of PMDF cultures inhibited HVP2ap replication but did not affect HVP2nv. The differential pathogenicity of HVP2 subtypes in mice and the lack of such differences in the natural baboon host are recapitulated in the primary dermal fibroblast cell culture system. This model may prove useful in examining early, local, host responses that influence the outcome of cross-species infections.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
£29.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (United Kingdom)

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • A Basu U Rodeck GC Predergast CC Howe (1999) ArticleTitleLoss of insulin-like growth factor I receptor-dependent expression of P107 and cyclin A in cells that lack the extracellular matrix protein secreted protein acidic and rich in cysteine Cell Growth Diff 10 721–728 Occurrence Handle10593648 Occurrence Handle1:CAS:528:DyaK1MXns1Ols7s%3D

    PubMed  CAS  Google Scholar 

  • JE Bigger DW Martin (2002) ArticleTitle Herpesvirus papio 2 (HVP-2) encodes a virion host shutoff function Virol 304 33–43 Occurrence Handle10.1006/viro.2002.1710 Occurrence Handle1:CAS:528:DC%2BD38XpsFyhtL8%3D

    Article  CAS  Google Scholar 

  • JE Bigger DW Martin (2003) ArticleTitleThe genome of Herpesvirus papio 2 is closely related to the genomes of human herpes simplex viruses J Gen Virol 84 1411–1414 Occurrence Handle12771408 Occurrence Handle10.1099/vir.0.19053-0 Occurrence Handle1:CAS:528:DC%2BD3sXks1Gmt78%3D

    Article  PubMed  CAS  Google Scholar 

  • R Eberle D Black (1991) ArticleTitleThe simian herpesvirus SA8 homologue of the herpes simplex virus gB gene: mapping, sequencing, and comparison to the HSV gB Arch Virol 118 67–86 Occurrence Handle1646593 Occurrence Handle10.1007/BF01311304 Occurrence Handle1:CAS:528:DyaK38XktVWqsbw%3D

    Article  PubMed  CAS  Google Scholar 

  • R Eberle D Black SL Lipper JK Hilliard (1995) ArticleTitle Herpesvirus papio 2, an SA8-like α-herpesvirus of baboons Arch Virol 140 529–545 Occurrence Handle7733825 Occurrence Handle10.1007/BF01718429 Occurrence Handle1:CAS:528:DyaK2MXlsVygu7g%3D

    Article  PubMed  CAS  Google Scholar 

  • R Eberle J Hilliard (1995) ArticleTitleThe simian herpesviruses Infect Agents Dis 4 55–70 Occurrence Handle7613729 Occurrence Handle1:STN:280:ByqA3sfksVw%3D

    PubMed  CAS  Google Scholar 

  • R Eberle DH Black TW Lehenbauer GW White (1998) ArticleTitleShedding and transmission of baboon Herpesvirus papio 2 (HVP2) in a breeding colony Lab Anim Sci 48 23–28 Occurrence Handle9517885 Occurrence Handle1:STN:280:DyaK1c7nslSltg%3D%3D

    PubMed  CAS  Google Scholar 

  • O Haller G Kochs F Weber (2006) ArticleTitleThe interferon response circuit: induction and suppression by pathogenic viruses Virol 344 119–130 Occurrence Handle10.1016/j.virol.2005.09.024 Occurrence Handle1:CAS:528:DC%2BD2MXhtlCqtbzK

    Article  CAS  Google Scholar 

  • JK Hilliard D Black R Eberle (1989) ArticleTitleSimian alphaherpesviruses and their relation to the human simplex viruses Arch Virol 109 83–102 Occurrence Handle2558632 Occurrence Handle10.1007/BF01310520 Occurrence Handle1:STN:280:By%2BC38%2Fps1A%3D

    Article  PubMed  CAS  Google Scholar 

  • JL Huff PA Barry (2003) ArticleTitleB-virus (Cercopithecine herpesvirus 1) infection in humans and macaques: potential for zoonotic disease Emerg Infect Dis 9 246–250 Occurrence Handle12603998

    PubMed  Google Scholar 

  • SA Keeble (1960) ArticleTitleB virus infection in monkeys Ann NY Acad Sci 85 960–969 Occurrence Handle13752136 Occurrence Handle10.1111/j.1749-6632.1960.tb50016.x Occurrence Handle1:STN:280:CC%2BD2s7ovFA%3D

    Article  PubMed  CAS  Google Scholar 

  • H Malherbe M Strickland-Cholmley (1969) ArticleTitleSiman herpesvirus SA8 from a baboon Lancet 294 1427 Occurrence Handle10.1016/S0140-6736(69)90972-6

    Article  Google Scholar 

  • AE Palmer (1987) ArticleTitle Herpesvirus simiae: historical perspective J Med Primatol 16 99–130 Occurrence Handle3035187 Occurrence Handle1:STN:280:BiiB38nhtFY%3D

    PubMed  CAS  Google Scholar 

  • JW Ritchey KA Ealey M Payton R Eberle (2002) ArticleTitleComparative pathology of infections with baboon and african green monkey alpha-herpesviruses in mice J Compar Pathol 127 150–161 Occurrence Handle10.1053/jcpa.2002.0575 Occurrence Handle1:STN:280:DC%2BD38vptlCntQ%3D%3D

    Article  CAS  Google Scholar 

  • KM Rogers KA Ealey JW Ritchey DH Black R Eberle (2003) ArticleTitlePathogenicity of different baboon Herpesvirus papio 2 isolates is characterized by either extreme neurovirulence or complete apathogenicity J Virol 77 10731–10739 Occurrence Handle14512523 Occurrence Handle10.1128/JVI.77.20.10731-10739.2003 Occurrence Handle1:CAS:528:DC%2BD3sXotFOgt78%3D

    Article  PubMed  CAS  Google Scholar 

  • KM Rogers RF Wolf GL White R Eberle (2005) ArticleTitleExperimental infection of baboons (Papio cynocephalus anubis) with apathogenic and neurovirulent subtypes of Herpesvirus papio 2 Comp Med 55 425–430 Occurrence Handle16270898 Occurrence Handle1:CAS:528:DC%2BD2MXhtFGqtbbK

    PubMed  CAS  Google Scholar 

  • KM Rogers JW Ritchey M Payton DH Black R Eberle (2006) ArticleTitleNeuropathogenesis of Herpesvirus papio 2 in mice parallels Cercopithecine herpesvirus 1 (B Virus) infections in humans J Gen Virol 87 267–276 Occurrence Handle16432011 Occurrence Handle10.1099/vir.0.81476-0 Occurrence Handle1:CAS:528:DC%2BD28XhtV2jsb8%3D

    Article  PubMed  CAS  Google Scholar 

  • Y Saegusa H Tabata (2003) ArticleTitleUsefulness of infrared thermometry in determining body temperature in mice J Vet Med Sci 65 1365–1367 Occurrence Handle14709829 Occurrence Handle10.1292/jvms.65.1365

    Article  PubMed  Google Scholar 

  • M Sato H Suemori N Hata M Asagiri K Ogasawara K Nakao T Nakaya M Katsuk S Noguchi N Tanaka T Taniguchi (2000) ArticleTitleDistinct and essential roles of transcription factors IRF-3 and IRF-7 in response to viruses for IFN-alpha/beta gene induction Immunity 13 539–548 Occurrence Handle11070172 Occurrence Handle10.1016/S1074-7613(00)00053-4 Occurrence Handle1:CAS:528:DC%2BD3cXnsl2nt78%3D

    Article  PubMed  CAS  Google Scholar 

  • JG Stevens (1993) ArticleTitleHSV-1 neuroinvasiveness Intervirol 35 152–163 Occurrence Handle1:STN:280:ByuD3M7otVI%3D

    CAS  Google Scholar 

  • SA Thompson JK Hilliard D Kittel S Lipper WE Giddens DH Black R Eberle (2000) ArticleTitleRetrospective analysis of an outbreak of B virus in a colony of DeBrazza’s monkeys (Cercopithecine neglectus) Comp Med 50 649–657 Occurrence Handle11200573 Occurrence Handle1:STN:280:DC%2BD3M3gt1SmsA%3D%3D

    PubMed  CAS  Google Scholar 

  • SD Tyler A Severini (2006) ArticleTitleThe complete genome sequence of herpesvirus papio 2 (Cercopithecine Herpesvirus 16) shows evidence of recombination events among various progenitor herpesviruses J Virol 80 1214–1221 Occurrence Handle16414998 Occurrence Handle10.1128/JVI.80.3.1214-1221.2006 Occurrence Handle1:CAS:528:DC%2BD28XpvVWhtA%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • BJ Weigler (1992) ArticleTitleBiology of B virus in macaque and human hosts: a review Clin Infect Dis 14 555–567 Occurrence Handle1313312 Occurrence Handle1:STN:280:By2B3cfhtl0%3D

    PubMed  CAS  Google Scholar 

  • RJ Whitley (2001) Herpes simplex viruses DM Knipe PM Howley (Eds) Fields virology Lippincott Williams and Wilkins Philadelphia 2461–2509

    Google Scholar 

  • M Yamada Y Arao F Uro S Nii (1986) ArticleTitleMechanisms of difference in pathogenicity between two variants of a laboratory strain of herpes simplex virus type 1 Microb Immunol 30 1259–1270 Occurrence Handle1:STN:280:BiiB3c7hsFQ%3D

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK, U.S.A.

    K. M. Rogers, D. H. Black & R. Eberle

Authors
  1. K. M. Rogers
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. H. Black
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Eberle
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rogers, K., Black, D. & Eberle, R. Primary mouse dermal fibroblast cell cultures as an in vitro model system for the differential pathogenicity of cross-species herpesvirus papio 2 infections. Arch Virol 152, 543–552 (2007). https://doi.org/10.1007/s00705-006-0865-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-006-0865-1

Keywords

Search

Navigation