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Determination of tetracycline using imprinted polymethacrylates along with fluorescent CdTe quantum dots on plastic substrates

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Abstract

Molecular imprints (MIPs) composed of CdTe quantum dots and tetracycline-imprinted polymethacrylates were synthesized on the surface of flexible plastic polyimide sheets from methacrylic acid, allyl mercaptan, and ethylene glycol dimethacrylate. The amount of aqueous CdTe solution, ethanol diluent, the reaction time and temperature of the radical-initiated polymerization were optimized. The MIP-QD composites were then copolymerized on the methacrylated polyimides. The resulting films were then immersed into a supersonic water-ethanol bath to strip off the tetracycline (Tc) templates. The fluorescence quenching intensity measured at 565 nm under 315 nm excitation before and after dropping a Tc sample on the film was used to optimize the processes. The calibration plot is linear in the 70 μM to 2.2 mM Tc concentration range at pH 7.5, and the LOD is 8.8 μM. The relative standard deviation is 8.2% (for n = 10). The method was applied to the determination of Tc in spiked samples of bovine serum albumin and fetal bovine serum and gave recoveries of 98% (at 200 μg⋅mL−1) and 97% (at 1.0 ppt), respectively.

Flexible polyimide substrates were chemically modified with methacrylate groups for anchoring the composites (MIP-QD) of tetracycline-imprinted polymers and CdTe quantum dots. The fluorescence of the integrated substrates is quenched by tetracycline but not by methacycline and steroids.

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Acknowledgements

Support for this work by the Ministry of Science and Technology under Grant no. MOST–105–2119–M–039–001 and the China Medical University under Grant no. CMU104-S-30 is gratefully acknowledged.

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  1. School of Pharmacy, China Medical University, No. 91 Hsueh-Shih Road, Taichung, 40402, Taiwan

    Jian-Lian Chen

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Correspondence to Jian-Lian Chen.

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Chen, JL. Determination of tetracycline using imprinted polymethacrylates along with fluorescent CdTe quantum dots on plastic substrates. Microchim Acta 184, 1335–1343 (2017). https://doi.org/10.1007/s00604-017-2118-2

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  • DOI: https://doi.org/10.1007/s00604-017-2118-2

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