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Optical determination of glucose and hydrogen peroxide using a nanocomposite prepared from glucose oxidase and magnetite nanoparticles immobilized on graphene oxide

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Abstract

Fe3O4 nanoparticles were deposited on sheets of graphene oxide (GO) by a precipitation method, and glucose oxidase (GOx) was then immobilized on this material to produce a GOx/Fe3O4/GO magnetic nanocomposite containing crosslinked enzyme clusters. The 3-component composite functions as a binary enzyme that was employed in a photometric method for the determination of glucose and hydrogen peroxide where the GOx/Fe3O4/GO nanoparticles cause the generation of H2O2 which, in turn, oxidize the substrate N,N-diethyl-p-phenylenediamine to form a purple product with an absorption maximum at 550 nm. The absorbance at 550 nm can be correlated to the concentration of glucose and/or hydrogen peroxide. Under optimized conditions, the calibration plot is linear in the 0.5 to 600 μM glucose concentration range, and the detection limit is 0.2 μM. The respective plot for H2O2 ranges from 0.1 to 10 μM, and the detection limit is 0.04 μM. The method was successfully applied to the determination of glucose in human serum samples. The GOx/Fe3O4/GO nanoparticles are reusable.

A one-step spectrophotometric method for the detection of glucose and/or H2O2 was developed by using GOx immobilized Fe3O4/GO MNPs as a bienzyme system and DPD as a substrate.

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Acknowledgments

This project was supported by grants from the National Science Foundation of China (Nos. 21077037 and 21107143) and the Fundamental Research Funds for the Central Universities, South-Central University for Nationalities (No. CZQ11019).

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Authors and Affiliations

  1. Key Laboratory of Catalysis and Materials Science of the State Ethnic Affairs Commission and Ministry of Education, College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan, 430074, People’s Repubic of China

    Qing Chang & Heqing Tang

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  1. Qing Chang
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  2. Heqing Tang
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Correspondence to Heqing Tang.

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Chang, Q., Tang, H. Optical determination of glucose and hydrogen peroxide using a nanocomposite prepared from glucose oxidase and magnetite nanoparticles immobilized on graphene oxide. Microchim Acta 181, 527–534 (2014). https://doi.org/10.1007/s00604-013-1145-x

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  • DOI: https://doi.org/10.1007/s00604-013-1145-x

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