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Enhancing polyhydroxybutyrate production from high cell density fed-batch fermentation of Bacillus megaterium BA-019

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Abstract

This study demonstrated the improved polyhydroxybutyrate (PHB) production via high cell density cultivation of Bacillus megaterium BA-019 with balanced initial total sugar concentration and carbon to nitrogen (C/N) weight ratio. In the 10 L stirred fermentor operated at 30 °C, pH 7.0, 600 rpm, and 1.0 vvm air, with the initial total sugar concentration of 60 g/L and urea at the C/N weight ratio of 10:1, 32.48 g/L cell biomass with the corresponding PHB weight content of 26.94 % and volumetric productivity of 0.73 g/L h were obtained from batch cultivation. Continuing cultivation by intermittent feeding of the sugarcane molasses along with urea at the C/N weight ratio of 12.5:1 gave much improved biomass and PHB production (90.71 g/L biomass with 45.84 % PHB content and 1.73 g/L h PHB productivity). Similar biomass and PHB yields were obtained in the 90 L stirred fermentor when using the impeller tip speed as the scale-up criterion.

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Acknowledgments

This work has been supported by the National Innovation Agency (NIA), Ministry of Science and Technology, Thailand. Partial funding from the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission (AM1026A-55) and Integrated Innovation Academic Center: IIAC Chulalongkorn University Centenary Academic Development Project is also acknowledged (CU56-AM05). The authors were thankful for sugarcane molasses sample provided by Mitr Phol Sugarcane Research Center, Thailand. The preliminary work done by Kusuma Kamolcharatsopa (2004) and Boonyarit Meksiriporn (2008) and the assistance on genetics work performed by Budsabatip Prasirtsak are highly acknowledged.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Pawut Kanjanachumpol & Songsri Kulpreecha

  2. Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok, 10330, Thailand

    Vasana Tolieng & Nuttha Thongchul

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  1. Pawut Kanjanachumpol
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  2. Songsri Kulpreecha
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  3. Vasana Tolieng
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  4. Nuttha Thongchul
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Correspondence to Nuttha Thongchul.

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Kanjanachumpol, P., Kulpreecha, S., Tolieng, V. et al. Enhancing polyhydroxybutyrate production from high cell density fed-batch fermentation of Bacillus megaterium BA-019. Bioprocess Biosyst Eng 36, 1463–1474 (2013). https://doi.org/10.1007/s00449-013-0885-7

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  • DOI: https://doi.org/10.1007/s00449-013-0885-7

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