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The presence and prognostic significance of human papillomavirus in squamous cell carcinoma of the larynx

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Abstract

The aim of the present study was to evaluate the role of HPV in laryngeal squamous cell carcinoma and correlate it with patients’ clinicopathological data. In total, 78 laryngeal squamous cell carcinoma patients enrolled in this study. The presence of genotype-specific HPV DNA was evaluated using Genotyping Assay in formalin-fixed paraffin-embedded tissue which was diagnosed between 2005 and 2015. All samples were also evaluated for p16 immunohistochemical staining. HPV DNA and p16 status were assessed in terms of location, smoking, alcohol consumption, lymph node status, tumor stage, overall survival, disease-free survival, perineural invasion, and vascular invasion retrospectively. Five test samples were excluded from the study due to inadequate deoxyribonucleic acid purity. HPV DNA was detected in 19 of 73 (26.02%) in patients with laryngeal squamous cell carcinoma. Human papilloma virus genotyping revealed double human papilloma virus in one case (types 16 and 59) and HPV 16 in the remaining cases. Although HPV-positive cases showed slightly better 3 years survival than HPV-negative ones, this finding was not statistically significant (overall survival p = 0.417, HPV positive: 92.3%, HPV negative: 81.4%, and disease-free survival p = 0.526, HPV positive: 93.8%, HPV negative: 80.9%). The presence of HPV DNA was not significantly associated with any clinicopathological features (p > 0.05). Among 73 patients, only 4 had an immunohistochemical staining of p16 and these patients were also HPV DNA 16 positive. Although our study results revealed a slightly better survival in patients with HPV DNA positivity for HPV 16 compared to the negative ones, the difference was not statistically significant. However, an increasing rate in especially high-risk-type HPV-16 prevalence in laryngeal squamous cell carcinoma by RT-PCR method was observed compared to our previous study. Although the presence of HPV in laryngeal SCCs seems to be associated with slightly better prognosis, additional studies may be needed, since our results were not statistically significant. We believed that HPV is not an adequate biomarker for diagnostic and prognostic purposes in laryngeal squamous cell carcinoma.

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Acknowledgements

Gulhane Military Medical Academy, Haydarpasa Training Hospital has been affiliated to the Health Science University and renamed as Health Science University, Sultan Abdulhamid Han Training Hospital Istanbul, Turkey.

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Authors and Affiliations

  1. Department of Otorhinolaryngology, Gulhane School of Medicine, University of Health Sciences, Sultan Abdulhamid Han Training Hospital, Istanbul, Turkey

    Evren Erkul

  2. Department of Pathology, University of Health Sciences, Sultan Abdulhamid Han Training Hospital, Istanbul, Turkey

    Ismail Yilmaz, Gizem Narli & Dilaver Demirel

  3. Private Practice, Ankara, Turkey

    Mustafa Alparslan Babayigit

  4. Department of Otorhinolaryngology, NB Kadikoy Hospital, Istanbul, Turkey

    Atila Gungor

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  1. Evren Erkul
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  2. Ismail Yilmaz
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  3. Gizem Narli
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  4. Mustafa Alparslan Babayigit
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  5. Atila Gungor
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  6. Dilaver Demirel
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Corresponding author

Correspondence to Evren Erkul.

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No conflict of interest was declared by the authors.

Human and animal rights

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. This study is a retrospective study, and for this type of study, formal consent is not required.

Financial disclosure

The authors declared that this study has supported financially for only HPV DNA Kits by our previous institution, Gulhane Military Medical Academy, Haydarpasa Training Hospital (27.10.2014-4013-266-14).

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Erkul, E., Yilmaz, I., Narli, G. et al. The presence and prognostic significance of human papillomavirus in squamous cell carcinoma of the larynx. Eur Arch Otorhinolaryngol 274, 2921–2926 (2017). https://doi.org/10.1007/s00405-017-4573-0

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  • DOI: https://doi.org/10.1007/s00405-017-4573-0

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