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Coenzyme Q10 content in follicular fluid and its relationship with oocyte fertilization and embryo grading

  • Reproductive Medicine
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Background

No data are available on the presence and content of Coenzyme Q10 (CoQ10) in human follicular fluid and its role.

Objective

To assess the presence and concentration of CoQ10 in human follicular fluid in relation to oocyte fertilization.

Methods

CQ10 content was measured in follicular fluid obtained from 20 infertile women undergoing ovarian stimulation program for in vitro fertilization. CoQ10 levels were assayed by high-performance liquid chromatography system and normalized for follicular cholesterol and protein levels. Oocyte morphology and embryo grading were assessed.

Results

CoQ10/Protein levels resulted significantly in mature versus dysmorphic oocytes. Similarly, CoQ10/Cholesterol was significantly higher in grading I–II versus grading III–IV embryos.

Conclusions

This study is the first demonstration of the presence of CoQ10 in the human follicular fluid. Although the biological and endocrine mechanism of CoQ10 in the follicular fluid and its correlation with oocyte and embryo development is unclear, a new step may be the administration of CoQ10 in infertile women to evaluate the biological and reproductive outcomes.

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Abbreviations

CoQ10:

Coenzyme Q10

IVF–ET:

In vitro fertilization and embryo transfer

SOD:

Superoxide dismutase

SeGPX:

Glutathione peroxidase

QH2 :

Ubiquinol

HPLC:

High-performance liquid chromatography system

ECD:

Electrochemical detector

References

  1. MacLeod J (1943) The role of oxygen in the metabolism and motility of human spermatozoa. Am J Physiol 138:512–518

    CAS  Google Scholar 

  2. Knapen MF, Zusterzeel PL, Peters WH, Steegers EA (1999) Glutathione and glutathione-related enzymes in reproduction: a review. Eur J Obstet Gynecol Reprod Biol 82:171–184

    Article  PubMed  CAS  Google Scholar 

  3. Paszkowski T, Clarke RN (1996) Antioxidant capacity of preimplantation embryo culture medium declines following the incubation of poor quality embryos. Hum Reprod 11:2493–2495

    PubMed  CAS  Google Scholar 

  4. Yang HW, Hwang KJ, Kwon HC, Kim HS, Choi KW, Oh KS (1998) Detection of reactive oxygen species (ROS) and apoptosis in human fragmented embryos. Hum Reprod 13:998–1002

    Article  PubMed  CAS  Google Scholar 

  5. Karlsson J (1987) Heart and skeletal muscle ubiquinone or CoQ10 as protective agent against radical formation in man. Adv Myochem 1:305–308

    Google Scholar 

  6. Ernster L, Forsmark-Andree P (1993) Ubiquinol: an endogenous antioxidant in aerobic organisms. Clin Invest 71:60–65

    Article  Google Scholar 

  7. Groneberg DA, Kindermann B, Althammer M, Klapper M, Vormann J, Littarru GP, Döring F (2005) Coenzyme Q10 affects expression of genes involved in cell signalling, metabolism and transport in human ***CaCo-2 cells. Int J Biochem Cell Biol 37:1208–1218

    Article  PubMed  CAS  Google Scholar 

  8. Kalen A, Appelkvist EL, Chojnaki T, Dallner G (1990) Nonaprenyl-4-hydroxibenzoate transferase, an enzyme involved in ubiquinone biosynthesis in endoplasmic reticulum–Golgi system. J Biol Chem 25:1158–1164

    Google Scholar 

  9. Alleva R, Tomassetti M, Battino M, Curatola G, Littarru G, Folkers K (1995) Role of CoQ10 in preventing peroxidation of LDL subfraction. Proc Natl Acad Sci USA 92:9388–9391

    Article  PubMed  CAS  Google Scholar 

  10. Mancini A, Conte G, Milardi D, De Marinis L, Littarru G (1998) Relationship between sperm cell ubiquinone and seminal parameters in subjects with and without varicocele. Andrologia 30:1–4

    Article  PubMed  CAS  Google Scholar 

  11. Balercia G, Arnaldi G, Fazioli F, Serresi M, Alleva R, Mancini A, Mosca F, Lamonica GR, Mantero F, Littarru GP (2002) Coenzyme Q10 levels in idiopathic and varicocele-associated asthenozoospermia. Andrologia 34:107–111

    Article  PubMed  CAS  Google Scholar 

  12. Veeck LL (1999) An atlas of human gametes and conceptuses: an illustrated reference for assisted reproductive technology. Parthenon Publishing, New York

    Google Scholar 

  13. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  PubMed  CAS  Google Scholar 

  14. Harvey MB, Arcellana-Panlilio M, Zhang X, Schultz GA, Watson AJ (1995) Expression of genes encoding antioxidant enzymes in preimplantation mouse and cow embryos and primary bovine oviduct cultures employed for embryo coculture. Biol Reprod 53:532–540

    Article  PubMed  CAS  Google Scholar 

  15. Caamano JN, Ryoo ZY, Thomas JA, Youngs CR (1996) b-Mercaptoethanol enhances blastocyst formation rate of bovine in vitro matured/in vitro fertilized embryos. Biol Reprod 55:1179–1184

    Article  PubMed  CAS  Google Scholar 

  16. Frei B, Kim MC, Ames BN (1990) Ubiquinol-10 is an effective lipid-soluble antioxidant at physiological concentrations. Proc Natl Acad Sci USA 87:4879–4883

    Article  PubMed  CAS  Google Scholar 

  17. Crane FL, Sun IL, Crowe RA, Alcain FJ, Löw H (1994) Coenzyme Q10, plasma membrane oxidase and growth control. Mol Aspects Med 15:1–11

    Article  Google Scholar 

  18. Stojkovic M, Westesen K, Zakhartchenko V, Stojkovic P, Boxhammer K, Wolf E (1999) Coenzyme Q(10) in submicron-sized dispersion improves development, hatching, cell proliferation, and adenosine triphosphate content of in vitro-produced bovine embryos. Biol Reprod 61:541–547

    Article  PubMed  CAS  Google Scholar 

  19. Kishi T, Okamoto T, Takahashi T, Goshima K, Yamagami T (1993) Cardiostimulatory action of coenzyme Q homologues on cultured myocardial cells and their biochemical mechanisms. Clin Investig 71:71–75

    Article  Google Scholar 

  20. Van Soom A, Boerjan M, Bols PEJ, Vanroose G, Lein A, Coryn M, De Kruif A (1997) Timing of compaction and inner cell allocation in bovine embryos produced in vivo after superovulation. Biol Reprod 57:1041–1049

    Article  PubMed  Google Scholar 

  21. Dumoulin JCM, van Wissen LCP, Menheere PPCA, Michiels AHJC, Geraedts JPM, Evers JHL (1997) Taurine acts as an osmolyte in human and mouse oocytes and embryos. Biol Reprod 56:739–744

    Article  PubMed  CAS  Google Scholar 

  22. De Sutter P, Dozortsev D, Qian C, Dhont M (1996) Oocyte morphology does not correlate with fertilization rate and embryo quality after intracytoplasmic sperm injection. Hum Reprod 11:595–597

    PubMed  Google Scholar 

  23. Balercia G, Buldreghini E, Vignini A, Tiano L, Paggi F, Amoroso S, Ricciardo-Lamonica G, Boscaro M, Lenzi A, Littarru G (2009) Coenzyme Q10 treatment in infertile men with idiopathic asthenozoospermia: a placebo-controlled, double-blind randomized trial. Fertil Steril 91:1785–1792

    Article  PubMed  CAS  Google Scholar 

  24. Chinnery P, Majamaa K, Turnbull D, Thorburn D (2006) Treatment for mitochondrial disorders. Cochrane Database Syst Rev 1:CD004426

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Acknowledgments

This research was funded by internal funds of the Marche Polytechnic University research grant.

Conflict of interest

All authors declare any financial and personal relationships with other people or organizations that could influence their work.

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Authors and Affiliations

  1. Department of Clinical Sciences, Unit of Obstetrics and Gynecology at “G. Salesi” Hospital, Polytechnic University of Marche, Via F. Corridoni 11, 60123, Ancona, Italy

    Angelo Turi, Stefano Raffaele Giannubilo, Silvia Battistoni, Fabrizia Santoni & Andrea Luigi Tranquilli

  2. Department of Biochemistry Biology and Genetics, Polytechnic University of Marche, Ancona, Italy

    Francesca Brugè, Federica Principi, GianPaolo Littarru & Luca Tiano

Authors
  1. Angelo Turi
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  2. Stefano Raffaele Giannubilo
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  3. Francesca Brugè
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  4. Federica Principi
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  5. Silvia Battistoni
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  6. Fabrizia Santoni
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  7. Andrea Luigi Tranquilli
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  8. GianPaolo Littarru
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  9. Luca Tiano
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Corresponding author

Correspondence to Stefano Raffaele Giannubilo.

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Turi, A., Giannubilo, S.R., Brugè, F. et al. Coenzyme Q10 content in follicular fluid and its relationship with oocyte fertilization and embryo grading. Arch Gynecol Obstet 285, 1173–1176 (2012). https://doi.org/10.1007/s00404-011-2169-2

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  • DOI: https://doi.org/10.1007/s00404-011-2169-2

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