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Mixed fermentation for natural product drug discovery

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Abstract

Natural products continue to play a major role in drug discovery and development. However, chemical redundancy is an ongoing problem. Genomic studies indicate that certain groups of bacteria and fungi have dozens of secondary metabolite pathways that are not expressed under standard laboratory growth conditions. One approach to more fully access the metabolic potential of cultivatable microbes is mixed fermentation, where the presence of neighboring microbes may induce secondary metabolite synthesis. Research to date indicates that mixed fermentation can result in increased antibiotic activity in crude extracts, increased yields of previously described metabolites, increased yields of previously undetected metabolites, analogues of known metabolites resulting from combined pathways and, importantly, induction of previously unexpressed pathways for bioactive constituents.

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Acknowledgments

Financial support was provided by grants R01 CA90441-01-05, 2R56 CA090441-06A1, and 5R01 CA090441-07 from the Division of Cancer Treatment, Diagnosis and Centers, National Cancer Institute, DHHS, and the Arizona Biomedical Research Commission. Many thanks to Dr. F. Hogan for preparing Fig. 1.

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  1. Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ, 85287-2404, USA

    Robin K. Pettit

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  1. Robin K. Pettit
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Correspondence to Robin K. Pettit.

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Pettit, R.K. Mixed fermentation for natural product drug discovery. Appl Microbiol Biotechnol 83, 19–25 (2009). https://doi.org/10.1007/s00253-009-1916-9

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  • DOI: https://doi.org/10.1007/s00253-009-1916-9

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