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Carbon-limited fed-batch production of medium-chain-length polyhydroxyalkanoates from nonanoic acid by Pseudomonas putida KT2440

  • Biotechnological Products and Process Engineering
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Abstract

Pseudomonas putida KT2440 grew on glucose at a specific rate of 0.48 h−1 but accumulated almost no poly-3-hydroxyalkanoates (PHA). Subsequent nitrogen limitation on nonanoic acid resulted in the accumulation of only 27% medium-chain-length PHA (MCL-PHA). In contrast, exponential nonanoic acid-limited growth (μ = 0.15 h−1) produced 70 g l−1 biomass containing 75% PHA. At a higher exponential feed rate (μ = 0.25 h−1), the overall productivity was increased but less biomass (56 g l−1) was produced due to higher oxygen demand, and the biomass contained less PHA (67%). It was concluded that carbon-limited exponential feeding of nonanoic acid or related substrates to cultures of P. putida KT2440 is a simple and highly effective method of producing MCL-PHA. Nitrogen limitation is unnecessary.

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References

  • Brandl H, Gross RA, Lenz RW, Fuller RC (1988) Pseudomonas oleovorans as a source of poly(beta-hydroxyalkanoates) for potential applications as biodegradable polyesters. Appl Environ Microbiol 54:1977–1982

    Article  CAS  Google Scholar 

  • Braunegg G, Bogensberger B (1985) About kinetics of growth and accumulation of poly-d(−)-3-hydroxybutyric acid in Alcaligenes latus strains. Acta Biotechnol 5:339–345

    Article  CAS  Google Scholar 

  • Braunegg G, Sonnleitner B, Lafferty RM (1978) Rapid gas-chromatographic method for determination of poly-beta-hydroxybutyric acid in microbial biomass. Eur J Appl Microb Biotechnol 6:29–37

    Article  CAS  Google Scholar 

  • Byrom D (1990) Industrial production of copolymer from Alcaligenes eutrophus. In: Dawes EA (eds) Novel biodegradable microbial polymers, Kluwer, Netherlands, pp 113–117

    Chapter  Google Scholar 

  • Carnicero D, FernandezValverde M, Canedo LM, Schleissner C, Luengo JM (1997) Octanoic acid uptake in Pseudomonas putida U. FEMS Microbiol Lett 149:51–58

    Article  CAS  Google Scholar 

  • Chen GQ, Wu Q (2005) The application of polyhydroxyalkanoates as tissue engineering materials. Biomaterials 26:6565–6578

    Article  CAS  Google Scholar 

  • Clesceri LS, Greenberg AE, Eaton AD (1999) Standard methods for the examination of water and wastewater, 20th edition, American Public Health Association, Washington, DC

  • Diard S, Carlier JP, Ageron E, Grimont PAD, Langlois V, Guerin P, Bouvet OMM (2002) Accumulation of poly(3-hydroxybutyrate) from octanoate, in different Pseudomonas belonging to the rRNA homology group I. Syst Appl Microbiol 25:183–188

    Article  CAS  Google Scholar 

  • Diniz SC, Taciro MK, Gomez JG, da Cruz Pradella JG (2004) High-cell-density cultivation of Pseudomonas putida IPT 046 and medium-chain-length polyhydroxyalkanoate production from sugarcane carbohydrates. Appl Biochem Biotechnol 119:51–70

    Article  CAS  Google Scholar 

  • Dufresne A, Samain E (1998) Preparation and characterization of a poly(beta-hydroxyoctanoate) latex produced by Pseudomonas oleovorans. Macromolecules 31:6426–6433

    Article  CAS  Google Scholar 

  • Durner R, Zinn M, Witholt B, Egli T (2001) Accumulation of poly[(R)-3-hydroxyalkanoates] in Pseudomonas oleovorans during growth in batch and chemostat culture with different carbon sources. Biotechnol Bioeng 72:278–288

    Article  CAS  Google Scholar 

  • Gross RA, Demello C, Lenz RW, Brandl H, Fuller RC (1989) Biosynthesis and characterization of poly(beta-hydroxyalkanoates) produced by Pseudomonas oleovorans. Macromolecules 22:1106–1115

    Article  CAS  Google Scholar 

  • Hänggi UJ (1990) Pilot scale production of PHA with A. latus In: Dawes EA (eds), Novel biodegradable microbial polymers, Kluwer, Netherlands, pp 65–70

    Chapter  Google Scholar 

  • Hazenberg W, Witholt B (1997) Efficient production of medium-chain-length poly(3-hydroxyalkanoates) from octane by Pseudomonas oleovorans: economic considerations. Appl Microbiol Biotechnol 48:588–596

    Article  CAS  Google Scholar 

  • Huijberts GNM, Eggink G (1996) Production of poly(3-hydroxyalkanoates) by Pseudomonas putida KT2442 in continuous cultures. Appl Microbiol Biotechnol 46:233–239

    Article  CAS  Google Scholar 

  • Huisman GW, Wonink E, De Koning G, Preusting H, Witholt B (1992) Synthesis of poly(3-hydroxyalkanoates) by mutant and recombinant Pseudomonas strains. Appl Microbiol Biotechnol 38:1–5

    Article  CAS  Google Scholar 

  • Jung K, Hazenberg W, Prieto M, Witholt B (2001) Two-stage continuous process development for the production of medium-chain-length poly(3-hydroxyalkanoates). Biotechnol Bioeng 72:19–24

    Article  CAS  Google Scholar 

  • Kang HO, Chung CW, Kim HW, Kim YB, Rhee YH (2001) Cometabolic biosynthesis of copolyesters consisting of 3-hydroxyvalerate and medium-chain-length 3-hydroxyalkanoates by Pseudomonas sp. DSY-82. Antonie Van Leeuwenhoek 80:185–191

    Article  CAS  Google Scholar 

  • Kim BS (2002) Production of medium chain length polyhydroxyalkanoates by fed-batch culture of Pseudomonas oleovorans. Biotechnol Lett 24:125–130

    Article  CAS  Google Scholar 

  • Kim GJ, Lee IY, Yoon SC, Shin YC, Park YH (1997) Enhanced yield and a high production of medium-chain-length poly(3-hydroxyalkanoates) in a two-step fed-batch cultivation of Pseudomonas putida by combined use of glucose and octanoate. Enzyme Microb Technol 20:500–505

    Article  CAS  Google Scholar 

  • Lageveen RG, Huisman GW, Preusting H, Ketelaar P, Eggink G, Witholt B (1988) Formation of polyesters by Pseudomonas oleovorans—effect of substrates on formation and composition of poly-(R)-3-hydroxyalkanoates and poly-(R)-3-hydroxyalkenoates. Appl Environ Microbiol 54:2924–2932

    Article  CAS  Google Scholar 

  • Lee SY, Wong HH, Choi JI, Lee SH, Lee SC, Han CS (2000) Production of medium-chain-length polyhydroxyalkanoates by high-cell-density cultivation of Pseudomonas putida under phosphorus limitation. Biotechnol Bioeng 68:466–470

    Article  CAS  Google Scholar 

  • Page WJ, Knosp O (1989) Hyperproduction of poly-beta-hydroxybutyrate during exponential growth of Azotobacter vinelandii UWD. Appl Environ Microbiol 55:1334–1339

    Article  CAS  Google Scholar 

  • Pouton CW, Akhtar S (1996) Biosynthetic polyhydroxyalkanoates and their potential in drug delivery. Adv Drug Deliv Rev 18:133–162

    Article  CAS  Google Scholar 

  • Preusting H, Vanhouten R, Hoefs A, Vanlangenberghe EK, Favrebulle O, Witholt B (1993) High-cell-density cultivation of Pseudomonas oleovorans—growth and production of poly (3-hydroxyalkanoates) in 2-liquid phase batch and fed-batch systems. Biotechnol Bioeng 41:550–556

    Article  CAS  Google Scholar 

  • Ramsay BA, Ramsay JA, Lomaliza K, Chavarie C, Bataille P (1990) Production of poly-(beta-hydroxybutyric-co-beta-hydroxyvaleric) acid copolymers. Appl Environ Microbiol 56:2093–2098

    Article  CAS  Google Scholar 

  • Ramsay BA, Saracovan I, Ramsay JA, Marchessault RH (1991) Continuous production of long-side-chain poly-beta-hydroxyalkanoates by Pseudomonas oleovorans. Appl Environ Microbiol 57:625–629

    Article  CAS  Google Scholar 

  • Ramsay BA, Saracovan I, Ramsay JA, Marchessault RH (1992) Effect of nitrogen limitation on long-side-chain poly-beta-hydroxyalkanoate synthesis by Pseudomonas resinovorans. Appl Environ Microbiol 58:744–746

    Article  CAS  Google Scholar 

  • Senior PJ, Dawes EA (1971) Poly-beta-hydroxybutyrate biosynthesis and regulation of glucose metabolism in Azotobacter beijerinckii. Biochem J 125(1):55–66

    CAS  PubMed  PubMed Central  Google Scholar 

  • Steinbuchel A, Lutke-Eversloh T (2003) Metabolic engineering and pathway construction for biotechnological production of relevant polyhydroxyalkanoates in microorganisms. Biochem Eng J 16:81–96

    Article  CAS  Google Scholar 

  • Sun Z, Ramsay JA, Guay M, Ramsay BA (2006) Automated feeding strategies for high-cell-density fed-batch cultivation of Pseudomonas putida KT2440. Appl Microbiol Biotechnol 71 (4):423–431

    Article  CAS  Google Scholar 

  • Timmis KN (2002) Pseudomonas putida: a cosmopolitan opportunist par excellence. Environ Microbiol 4:779–781

    Article  Google Scholar 

  • Weatherburn MW (1967) Phenol–hypochlorite reaction for determination of ammonia. Anal Chem 39:971–974

    Article  CAS  Google Scholar 

  • Williams SF, Martin DP, Horowitz DM, Peoples OP (1999) PHA applications: addressing the price performance issue: I. Tissue engineering. Int J Biol Macromol 25:111–121

    Article  CAS  Google Scholar 

  • Zinn M, Witholt B, Egli T (2001) Occurrence, synthesis and medical application of bacterial polyhydroxyalkanoate. Adv Drug Deliv Rev 53:5–21

    Article  CAS  Google Scholar 

Download references

Acknowledgement

This research was supported by the Natural Science and Engineering Research Council of Canada and a grant from BIOCAP Canada.

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Authors and Affiliations

  1. Department of Chemical Engineering, Queen’s University, Kingston, ON, K7L 3N6, Canada

    Zhiyong Sun, Juliana A. Ramsay & Martin Guay

  2. Polyferm Canada Inc., RR1, Harrowsmith, ON, K0H 1V0, Canada

    Bruce A. Ramsay

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  1. Zhiyong Sun
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  2. Juliana A. Ramsay
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  3. Martin Guay
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  4. Bruce A. Ramsay
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Correspondence to Bruce A. Ramsay.

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Sun, Z., Ramsay, J.A., Guay, M. et al. Carbon-limited fed-batch production of medium-chain-length polyhydroxyalkanoates from nonanoic acid by Pseudomonas putida KT2440. Appl Microbiol Biotechnol 74, 69–77 (2007). https://doi.org/10.1007/s00253-006-0655-4

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  • DOI: https://doi.org/10.1007/s00253-006-0655-4

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