Abstract
Staphylococcus epidermidis has emerged as one of the major nosocomial pathogens associated with infections of implanted medical devices. The most important factor in the pathogenesis of these infections is the formation of bacterial biofilms. Bacteria grown in biofilms are more resistant to antibiotics and to the immune defence system than planktonic bacteria. In these infections, the antimicrobial therapy usually fails and the removal of the biofilm-coated implanted device is the only effective solution. In this study, three proteomic approaches were performed to investigate membrane proteins associated to biofilm formation: (i) sample fractionation by gel electrophoresis, followed by isotopic labelling and LC–MS/MS analysis, (ii) in-solution sample preparation, followed by isotopic labelling and LC–MS/MS analysis and (iii) in-solution sample preparation and label-free LC–MS/MS analysis. We found that the commensal strain S. epidermidis CECT 231 grown in biofilms expressed higher levels of five membrane and membrane-associated proteins involved in pathogenesis: accumulation-associated protein, staphylococcal secretory antigen, signal transduction protein TRAP, ribonuclease Y and phenol soluble modulin beta 1 when compared with bacteria grown under planktonic conditions. These results indicate that a commensal strain can acquire a pathogenic phenotype depending on the mode of growth.
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Acknowledgments
S.A-A thanks the Basque Government and the Medical Research Council for supporting her stage in Cambridge. We also thank Eneritz Bilbao and Joe Carroll for their excellent technical assistance. The authors acknowledge the Analytical and High-Resolution Microscopy in Biomedicine Service at the University of Basque Country for assistance with Scanning Electron Microscopy. The Proteomics Core Facility-SGIKER (member of ProteoRed-ISCIII) is supported by Universidad del País Vasco/Euskal Herriko Unibertsitatea, Ministerio de Ciencia e Innovación, Gobierno Vasco/Eusko Jaurlaritza, and European Science Foundation. This work was supported by funds from the Spanish Ministry of Economy (Grant No. BFU2012-36241) and from MICINN (Grant No. BFU2010-22103). S.A-A was a pre-doctoral student supported by the Basque Government and by the Fundación Biofísica Bizkaia. We thank Prof. Holger Rohde from the Institut für Medizinische Mikrobiologie, Virologie und Hygiene (Hamburg, Germany) for supplying the anti-Aap antiserum.
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Águila-Arcos, S., Ding, S., Aloria, K. et al. A Commensal Strain of Staphylococcus epidermidis Overexpresses Membrane Proteins Associated with Pathogenesis When Grown in Biofilms. J Membrane Biol 248, 431–442 (2015). https://doi.org/10.1007/s00232-015-9801-1
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DOI: https://doi.org/10.1007/s00232-015-9801-1