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Effect of syringic acid and syringaldehyde on oxidative stress and inflammatory status in peripheral blood mononuclear cells from patients of myocardial infarction

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Abstract

Oxidative stress and inflammation are considered as therapeutic targets in myocardial injury. The aim of the present study was to investigate the protective effect of syringic acid (SA) and syringaldehyde (SYD) on peripheral blood mononuclear cells (PBMCs) of myocardial infarction (MI) patients. PBMCs from MI patients were cultured in the presence and absence of SA and SYD. The level of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and nitric oxide (NO) was estimated. Reactive oxygen species (ROS) formation, oxidation of lipids, proteins, and activity of antioxidant enzymes were also quantified. To further determine biomolecular changes in treated PBMCs, Fourier transform infrared (FTIR) spectroscopic analysis was done. Molecular docking study was also conducted to evaluate the binding interaction of SA and SYD with various target proteins. SA and SYD treated PBMCs of MI patients showed decreased secretion of TNF-α, IL-6, and NO. Moreover, the content of ROS, level of lipid, and protein oxidation showed diminution by treatment with both the compounds. Enhanced antioxidant defense was also observed in treated PBMCs. The FTIR spectra of treated cells revealed safeguarding effect of SA and SYD on biomolecular structure. The molecular docking analysis displayed significant binding affinity of the two compounds towards TNF-α, IL-6, and antioxidant enzymes. Our findings demonstrated potent antioxidant and anti-inflammatory effects of SA and SYD on PBMCs of MI patients. Thus, SA and SYD supplementation might be beneficial in attenuating oxidative stress and inflammation in MI.

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Acknowledgments

The authors extend their sincere appreciation to the research facility provided by Chairperson, Department of Biochemistry, Department of Physiology, Jawaharlal Nehru Medical College and University Sophisticated Instrumentation Centre, Aligarh Muslim University.

Authors contribution statement

SS, S Moin, SM: conceived and designed research. SS: conducted experiments. TK, AH, SSN, MA, AFF: contributed new reagents and analytical tools. SS, S Moin, TK, SMN: analyzed data. SS: wrote the manuscript. All authors read and approved the manuscript.

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Authors and Affiliations

  1. Department of Biochemistry, Jawaharlal Nehru Medical College, Faculty of Medicine, Aligarh Muslim University, Aligarh, Uttar Pradesh, India

    Sumayya Shahzad, Somaiya Mateen, Minhal Abidi, Abul Faiz Faizy & Shagufta Moin

  2. Department of Chemistry, Faculty of Science, Aligarh Muslim University, Aligarh, Uttar Pradesh, India

    Tasneem Kausar & Shahid M. Nayeem

  3. Department of Pharmacology, Jawaharlal Nehru Medical College, Faculty of Medicine, Aligarh Muslim University, Aligarh, Uttar Pradesh, India

    Syed Shariq Naeem

  4. Centre of Cardiology, Jawaharlal Nehru Medical College, Faculty of Medicine, Aligarh Muslim University, Aligarh, Uttar Pradesh, India

    Asif Hasan

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  1. Sumayya Shahzad
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Correspondence to Shagufta Moin.

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The study protocol was approved by the Institutional Ethics and Research Advisory Committee.

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Shahzad, S., Mateen, S., Kausar, T. et al. Effect of syringic acid and syringaldehyde on oxidative stress and inflammatory status in peripheral blood mononuclear cells from patients of myocardial infarction. Naunyn-Schmiedeberg's Arch Pharmacol 393, 691–704 (2020). https://doi.org/10.1007/s00210-019-01768-2

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